XB-CLONE-4353717
Clone Name: IMAGE:7026277
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Gene:
tut7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783436
[+]
taattaatccccccccccccaggagccaaggagctaggtattatttgtttgtgggagctgaagcagctaaccgcgtctgtcccaaagcggaactattgga
cggtatggataaggtgcggtgtctaattgtagcccataactgaatctctcataaagcattaatgattttgtctgcaatttgaaaaatgggggatgctgcc
aaacaagtcaaacaccatgcaataatggatgaagaagaatacaaaaggcctgttgtcaaacagtcaattggaaaccacgaaatacatggagttaagaaaa
tacaagtggactctcaaagaaagccaattccggtcatctgcagcaacagtcccagaaatgggctacatcagagcacttttaagaattacagtcgcagtcc
taatagccgaccaagcaatattcataaagatggacataagagatggcttaccgacgttcctcgtagtcactctgataactggagagatcggaagcaccag
ccatttaatcgaacaaggaacgattccaccaatgagcaagaagaggctcagaactggaggcgtgatggagacacttttaaacaggagaagaaaagatttg
acagaagaagatgtaaatctgagcgagatgaagaagattatattgagagccctgttatagatgaatcagctctatctcagagggacttgttgggcttgnc
aacagcagaagagcgtctaaaaagagactgcatttacaggctcaaaaggcgtccacggaaaataccccactgctggtttattgctgtaaagcttttgcga
tgcctttggtttgaa
3' EST: CF783437
[+]tttttttttttttttcctcagatttctcttatgccttttttctttaatatgcttatgagcagatgacacagactcaaccaaagcatcgcaaagcttacag
caataaacagcagtggggtatttccgtggacgccttttgagcctgtaaatgcagtctctttttagacgctcctctgcttgttgcaagcccaacaagtcct
tctgagatagagctgattcatctataacagggctctcaatataatcttcttcatctcgctcagatttacatcttcttctgtcaaatcttttcttctcctg
tttaaaagtgtctccatcacgcctccagttctgagcctcttcttgctcattggtggaatcgttccttgttcgattaaatggctggtgcttccgatctctc
cagttatcagagtgactacgaggaacgtcggtaagccatctcttatgtccacctttatgaaaaattgcttggttcggctattacgactgcggactgtaat
ttcttaaaaagtgcctctgaatgtaaaccccatttttctgggaactggttgcttgcgagaaggaaccggaaattgggccttttccttttggaaaagtccc
accttgtgtaattataccgtaaagtcccgatggttatttatcctggggttttccccaattaggaagtgttgttggaacaaaccagggccccttttgggaa
gtgtcattccttccaccccctttattttgccattgggtggttttgaaacccaggtgaggggagaagaatcccccccattttgttacaacaatagcaaaaa
atcaaaatgtttttaaggggccttattgggaaaaaaatttcagggttgatggggccctacaaaatttaaatcgacccggttcacgtttaaacccaataac
agcgtcgatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: