XB-CLONE-4353714
Clone Name: IMAGE:7026276
|
Gene:
fth1.1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783439
[+]
taattaatcccccccccccccgaacctcctctctggctctgagtagagttcttgcttcaacagtgtttgaacggaaccctctctgagtcttttttagacc
aaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcgtcagaa
cttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgatcgtgat
gacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaatgtgggg
gccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaagactgtgaa
ccaggctttactggatctgcacaagctggcatctgacaagactgattctcatctctgccactttctggaatctggagacttggagcaacacgtgaaggcc
atgaacgaccttggagaccacatcacaaacctgaatccgccttggggtgccccataaacgcatgggcgaataacttgttttgacaaacaccaccctgggg
ggagagtaagcctaaagcgcaccccccaaggggtggcatacccacctatattcgcctcctttcggttctccaaaaattactggttcctccttttacaaaa
ccttgttgttgggcactttatagctttttttgccgtccccccattgacactccccg
3' EST: CF783438
[+]tttttttttttttttggaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttat
tcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctg
actggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgcttgtcaaacaggtactcgcccatgccgttctgcggcaccccaaggcg
cttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttg
tcagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcac
gctctggtttcttgatatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaactttttcggcatgctccctttcctcatgactctgct
cccttgagaacttggccacatggtgggagtggcacgtcatcacgatcgaaatagtaagaacctggacagatagacntaggaggcatacaacttcatgttc
accataacggtggatggcggctccccaatccctgtttaaagttctgaaccacctggggatgcatggccgcccttttcggggtttgctggtgggcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: