XB-CLONE-4353711
Clone Name: IMAGE:7026275
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783440
[+]
taattaatcccccccccccccgatcccagggaccctgagaagaacaagatgaaagccattatcctgctcgctctgctagccgccgtcctgattcaggata
taagtgcagctccggtgcagcctgagggaacagagcctaagagtcctcttcaccgtagaggttgcgttatctggtcctgctttggaaaaagagatacaga
caaacctgaggcaaatggggaaggtgaagctctggtgaaacctgaggaaactgatataagtgcagctgcggtgcagcctgagggaacagatataagtgaa
gctctggagcagcctgaggaagctgagcataagagtcttcttcaccgtagaggttgcgttatctggtcctgctttggaaaaagagatacagaccaacctg
aggcaaatgtggaaggtgaagctctggtgaaatctgatgaaactgatataagtgcagctccggtgcagcctgagggaacagagcgcaaaatccgtcgtcc
cgttcgatctcttgctaattaagaccctatgccaaccggggattagaaccttcaacaaatgttgatgtgatttcagatgtgaagctaaggaaaagcctga
gccatggactctaaatgtttattttccattaaattttctcaactcctaaatattagttcatctccctttcctaatgtgagtcgtgtccttatccccggca
tgccgcgccttattcctttatggcccctgataacctctttatctttctttttaactgccctgacccccttccagagggatttggggtttttttgtcctga
aaaaacaacaat
3' EST: CF783441
[+]tttttttttttttttcactctaatgatgtctctttattgatatggtaacaatagcatcaaattgtatgtatttttcattgtgtttatcaagacaaaaaac
ccaaatcccttggaaggggtcaggcagataaaaagaaagataaagaggtatcaggggccataaaggaataaggcgcggcatgccggggataaggacacga
ctcacattaggaaagggagatgaactaatatttaggagttgaggaaatttaatggaaaataaacatttagagtccatggctcaggcttttccttagcttc
acatctgaaatcacatcaacatttgttgaaggttctaatccccggttggcatagggtcttaattagcaagagatcgaacgggacgacggattttgcgctc
tgttccctcaggctgcaccggagctgcacttatatcagtttcatcagatttcaccagagcttcaccttccacatttgcctcaggttggtctgtatctctt
tttccaaagcaggaccagataacgcaacctctacggtgaagaagactcttatgctcagcttcctcaggctgctccagagcttcacttatatctgttccct
caggctgcaccgcagctgcacttatatcagtttcctcaggtttcaccagagcttcaccttccccatttgcctcaagtttgtctgtatctcttttttcaaa
gcaggaccagataacgcaacctctacggtgaagaggactcttaggctctgttcccctcagctgcacccggagctgcactttatatcctggaatcaggacg
ggcggctaagcagagcgagcaagaataat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: