XB-CLONE-4353708
Clone Name: IMAGE:7026274
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Gene:
hnrnpa0
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783443 [+]
taattaatcccccccccccgtaagagtcttaaggcttttgtgacgtaagaaagaagagcagctgctacttccctctcctcctttcgccaccagataaaac
ggagagagagcgcctgagcgcgacgatctgtgcttgcgtttcaatgagtaccggcgatatggatacgacgcaactctgcaaactgtttatcggggggctt
aacgtgcagaccaccgaagagggcctacgccagcattttgagacctacggtcaactgacggactgcgtggtggttatcaacccacagaccaagcggtctc
gatgtttcggtttcgtaacctactcgagtgccgcggaggccgacgcggcggtggaggcctcgccccacgtggttgacggtaacaacgttgagctgaagcg
ggccgtctccagggaagattccgccaagccgggggcacacgctaaagtgaagaagctctttgttggcggcctgaaagaggacgtcggcgagagtgacttg
ctcgaacacttcgcccagtttggcgccgtggaaaaagtggagatcattgccgataaagtgtccggcaagaaacgcggcttcggctttgtgtactttacca
accacgattccgccgacaaggccgcggtggtcaagttccactccatcaacgggcaccgcgtcgaagtaaagaaagccgtgcccaaggaggaactttccca
gggtttccaatagatcctttccgcgggccgcaaggggcggggaaaggcccaaggntggccggcgggaacgccccccgggaactaccattggccatcccct
gggaaagtgggcgggccaaagcttgtctttttcgggggggggggggcta
3' EST: CF783442 [+]tttttttttttttttatttttttttttttttttttttttttaggggaacaaagtgggtaataaaccgatttatttaaaaagtaaaaacaaaaaagggtac
agggggtactgtttgcttgcagcagcccaacaggctggccaaaaacaaaaaaccagggtgggtgtgggaaaatacggcttccgtaactgccattgtcagt
ttcccgctctagatctcaggttacaatagtataaaaacaatcagagcccaaaacacacatgaaaatatgaaagaagttcgagtttatcggtgtgtttgtg
tggattatttccatctctcactgcgccaaagttattaaacctttcaaaaggagccgccgccataggagctgccgacgccataacctcctcctccgccgcc
gccgctataacctccgcggtaggggcctgacgtgttgggccggttccagctgccgcttcccgctcccttcatggacccgtacgaagactggtgctggctg
taactcccgaaactgttgtagccattgccgtagtcgctctgctcgccatagccgcctccgtacgagccgtaaccctggttgccgcccccatagccgccgt
agcccccaccgaaagcagctttgccgccacttccaaggatgccattgtagtcccggggcgctccgccgccaacctggcctctcccgcccctgccgcccgc
ggaaggatctattggaacccctgggaaaagttcctccttgggcacggccttcctttaactccaacgcggtgcccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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