XB-CLONE-4353702
Clone Name: IMAGE:7026272
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Gene:
rps28
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080501 [+]
CCGCTCTCTCTGCCTCCTACCGCCATCATGGAAGCCAGCCGCGTGCAGCCTATCAAGCTTGCCCGGGTAACAAAAGTCTTGGGACGCACAGGTTCTCAGG
GTCAGTGTACCCAGGTCCGTGTTGAGTTCATGGATGACAGCAACCGTTCCATCATTCGTAATGTTAAGGGACCAGTTAGAGAGGGGGATGTCCTGACTCT
GCTGGAATCTGAGCGAGAAGCTAGGAGATTACGTTGATTCTCACCTGGTCCTTGGCTTTCTTTACATAGAAGAAAAAACTTCAGTATGTACTATCGCTGG
ATAGTGGGCCATCTGTGAATCCAGACAAGATCTTGGTTTTCTGTTTGTAAAATAAAATTTGTAAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF783447 [+]taattaatcccccccccccccccgctctctctgcctcctaccgccatcatggaagccagccgcgtgcagcctatcaagcttgcccgggtaacaaaagtct
tgggacgcacaggttctcagggtcagtgtacccaggtccgtgttgagttcatggatgacagcaaccgttccatcattcgtaatgttaagggaccagttag
agagggggatgtcctgactctgctggaatctgagcgagaagctaggagattacgttgattctcacctggtccttggctttctttacatagaagacaaaac
ttcagtatgtactatcgctcgatagtgggtcatctgtgaatccagacaagatcttggttttctgtttgcaaaacaaaatttgttaacgaacgtattcccc
ctcttttatccccccccctatcctttatccgttctacctttccttaccattttttacactttcttcccatactctactatactgtacaatttttttttcg
caggggtccccccattttttttgagacgcggcgcgccccccccccggggtgaaatttttgtcctccgggccctaaaaattcggatctttttgtgaaaccc
aaacaaaaaggcttttaacaaaatataagacgccctctttcccgctccctacataaattttttcacccaataatttgtacccctatttttttttttttcc
gcgccactcgttttctaatttatcgcccctattttttttccaaaaacccctccccttttgcggacccttcattataaatagctccccgaggatcatgggg
aaaacattaatttctggcccccaaacaaactttgtgcccattaaaaatccaccaccccgccaataatacggtatattcgggcgataaagcgtctcttctt
tcgacaatatggaaaaaagaataacccccttgttgttcacccccgtgtttttattcttctttaggcccccgctaaaatatccctcttgcgcctcgcccat
atttt
3' EST: CF783446 [+]ttttttttttttttccctttttttttttttttttttttttttttttttttttggggttttttttttttttttttttttttttttttttttttttttnggg
cccntacaaattttttttttcaaaaaaaaaaccaaaattttttttgggtttccaaagggccccctttcccccgaaagaacaaaccgaagttttttttttt
ttttaaaaaaaccccagggccccgtgaaaaaccacctaatcccccaactttccccccaaattcccccaaaatcggggaatccccccctctaaaggggccc
ttaaaatttcaaaagagggaacggttttttttttccctgaaccccacccggacctgggtaccctgaccccgaaaacctttgggtcccaaaaatttttttt
ccccggcaaacttttaaaggttccccccggtggttttctttatgggggtaaaaagcaaaaaaaaccggggggggggggggggggaaatattataattccc
gggggcaaaaaaaaaaattattttttttttctggcccccgaaaaaaacccaagggtgcctttggacacccgggaaggggaacccctttgtggaaaaaaaa
gccacaaggggaattttttttctttaagggtgccccccaaaacccaattggtgtataaacacacaaagggtttttgtttttaattttgttcccgccgttt
tttaaaaaacaccaagggggggggggcgccttaaacactctttagggggggaaattttaagcccccctttggggcccgggggaaaaaccccgttttttaa
aggagaccctttccccccccccccaaaaacttggaatttcctcccccgggttttttcttttggggggggtcaaaaaaaccctgttggaagggctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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