XB-CLONE-4353699
Clone Name: IMAGE:7026270
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Gene:
ctnnb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783449 [+]
taattaatcccccccccccccccgctgtgtgtcgcgtttcaagcgaacgggagagtagtgcgtttgttcatggctgggaactgaaggggatcagaaacat
aaacggggaaaagaaaatcaacgctcttgccgccataccccgtccatcttaaagtgggacacaattcggaacctgcagggtctggaggtgtgtaggagac
ggccagtaccgggcgaaacgcggtgacggccggaaccggtggacgtcgagccaacggcttgtaatccacaacctggttctttggaaactgttgaaaaatg
gcaactcaagcagatctaatggagctggacatggccatggagccagatcggaaagcagcagtcagtcactggcagcagcagtcttacctggattctggta
tccattctggagcgaccaccacagcaccatctttaagtggcaaaggaaacccagaggatgaagatgtggacaccaaccaagttttgtatgaatgggagca
aggtttctctcagtcctttactcaagatcaagtggctgatattgatggccaatatgctatgacaagagcccagcgagttcgtgctgcaatgtttccagaa
acccttgatgaaggcatgcagattccatcaacgcaatttgactctgcacaccctacaaatgtgcaacgcttagcagaaccttcccagatgcttaagcatg
ctggggggcaacttgattcaatttaccaagatggatgcctgaacttgggctactcccaagcaattccccggaggcctggaaaaaaaatttcccttcaatg
gattcaagggacccaaggctttcgtattttt
3' EST: CF783448 [+]ttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttggggggcccccg
aaaaaaaaaaaatttttttttttttttttttttaaaaaaaaggggggccccccccaaaaaacccaaaacccccccccccggggggttttaaaaacccccc
ccccggggggttaaacccccccctttttttccccggccccttttttttgggggttttttttaacccggggggggggccccaaaaaatttggggggaaaaa
ggggcggggggaaaccccccccccccggcccccaaaaagggggtttttttggggccccccccccccatttcccaaaaaaattttttttaaaaaaaaggcc
ccccccgcttttttccccctttaaaaaaaaaaacgtgggggggccccccctggggggccttttttttcaaaaagggaaaaaaaaaaaaaagggggggggg
ccccccccggggtccctttttttggggggccccccgggggggggaaaaaatggggggcccccccggtgccaaaaaaaaaaaaaaccccccctttttttgt
ccccccgggggccccccaaaaaattgggggggcccctttgggggggaaaaaaaattggggggttttaaaaaacccccgggggggggcccccccccccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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