XB-CLONE-4353679
Clone Name: IMAGE:7026263
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783462
[+]
taattaatcccccccccccccccggcttgtggactggcgagcaaggaactgcataacattgttttagaagacttacggtatggtcatccaaattttggga
aaaaaccttattcggaaaaactttggtcccagacattcaagataactaatcttttgtttggatttggaagaaattaaccaatgaccttttattttacgca
actggctagagtgtgcaagcaaccaacgaattggtccaataggaaaaaagtaagggcaaagcctggacgtcatgatgccatcatctcacttgtgcagaga
ggataccataagtttcatacagtccttgtaccttgtttgcctctttccacttccgtgcttcctgttaccatcctcaggcaagcctccggtagtgtgggac
gtttgtgggcttgcttacatcgatttgtcttcctggccgtgacagtataaccaagccatggacagggaggaagaaccttctcctctctttacccttgccc
agcaaatcgcttccctgacacaggcagcgagagagcttcagggtggatatagccaagtcaaggaacaacaacgcaccttgcaaccaccggtacctggtct
ggttcctccttctcccactgctgctgctccggcagcccctgaacctaaagtggccatgcctgaaaagttttctggtaaccgaaagactttttgaaccctt
tactaacgcttgcaatctcctgtttttctcccaaatctagaatgtactcccagtggacaaatccaaagtgggggaggtggtttcctcccccttccggggn
ggaaccccccagttca
3' EST: CF783461
[+]tttttttttttttttcgtaatgagctttatttagggtgagtgacaaaggaatggattcatgcaaaatgggaccagagcttataggagaaccatcagcaac
tcgtaggaaaaccgggaattatttcttctttttttttaatttgattaatctctgcaactcgagagtcaagaaacacccactagctccagaattaagtatg
gctggaaggaatagagtcttgtttcccaactgcaaagaaatggacaaaaataataacggagcaaacttgtattggagagaatcagtctttaagataggat
ccttacacggacaagtaggacagttacggagcaggtgaccagcaagtccacaataaagacagagcttgagccgtctgcaacgtaaacgctcttccggaga
gaaagcggttcgcagagccccaatttgcatgggttcggactcatcagcaaccgaagtacctggttccgggcgaatgggtggtggagccttgagtaccagc
caagacgggtagaaatagccagcattttcaatttgaatagacagatgaatcagttcatctaaagtggctgggacccctactcgagccagctcatccttta
ggctgcaggaaagtcccaagcgatattgatgttttaaagctgcttgattccaatccacgtccgaggcgtattttcggaattccccagtgtagtcctcgac
aggtctggtcccttggtgaaaatttcttaaagctgcttcggctgtgggagtacgatgggggacccatcatataaaactgccaaaggcttggac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: