XB-CLONE-4353676
Clone Name: IMAGE:7026262
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Gene:
reep4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783464
[+]
taattaatccccccccccccccgcctcctgtacaggaagtgtatccatcctactctgtccttgaaggagaaggaaatcgattcctacattatacaggcaa
aagaacgaagttacgagtcatttgtgaacattgggcgaaaaggtttaaacatagcagccagtgcggcagtgcaagctgctaccaagggtcagggcgcatt
agtggggcgcctgcggagtttcagcatgcaggatttgcgtgctcttcctgatgacacacctatacactacagagatgccctgtatcctgatgcaccagag
ctgcaccggaggccaattggatacccaaccacttcccatgctgatagtgactccatggatgaacggtggtcggattctgaaatggccgaaacaaggacag
cagctaggacaagaggaggcatgccatctaaaccactacagaggagccagagtctgcgggtttccaagaagaaagggctaagtcgagaggtctctacgaa
aacaacgaaacccaaaggaaagaagaagcctgcacaatcagaacccgaaaactaaaccgggctttcaatgactgactgagggctgcgcctcgctgtaatc
gttctatggagcaaaaacactgaattttatggatgccaggatgtccctcactcagcatgatttctggggtgcaggattcacggcaacctgttgtggaaac
gacagtttgcgggtgaaagtgtgtgtgtgtatatattaaagtgtgaggtattaacccatgcagggccttctgtggcaactgctggatgccaagtttcctg
gcccccctttgcccccgggtttgcccccccgggttataaaaaaaaacacaacccaccttgtggtgggggtgttcggggcgaaagc
3' EST: CF783463
[+]tttttttttttttttattggcagagagtggtttatttatttgcatatcaaaacacagagattgtatataagcattaggcactttgcttggccccagataa
gaccaaagtgattggagggcccaaactgaaagctacattgtaccttggtcacttgatgcccatgagtggcactgtcccataaggtttcagcagaagtgga
atctttggcacttggggacatagcctttggctttgctggcccacttgaatgagtatccaagcaatgctatatacacatgtactgagcgacaaacatctat
ataaataggatcaagggcttgtgccctagtgcagagagagggaggcgcgataggaacatgatggcttgtacaccctacatcacgcggcagccaattcatt
ggctttaaactatcatcaacacacggaacaaacatttctacgaaaaaatatttccgttaaaaacacataaatctcaagtaataaaaaaaaaagaggaaac
gggagggttacggtcagttaagggccccacccaccaacaatatacagaacaggtttctattgggtgctggggagagggccttcagttgttgctgggagtt
gtaactaccgtatggcgagtcacagtgcggccatttgggctgtaagacattatatttaaccccataaaaacttaatgtacaattgcttgagagagttctt
tctgaacaactgttacaattttgcagggaaattttcctatacactgaatccacaattaattaaaaaattttttcccctaaataaccatcccccattaatt
at
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: