XB-CLONE-4353673
Clone Name: IMAGE:7026261
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Gene:
ttr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783466 [+]
taattaatcccccccccccccatattgtgcacaattgcatactctgaacaagaatggcttttttcaagtcttttcttctcttagccctactggcaattgc
ctcggaggctgcaccgggacatgtttcccacggtgaagctgactccaagtgcccccttatggtaaaagtattggatgcagtgagagggatccccgcagcc
aatctgctagtgcaagttttcaggaatacagagggaaactgggagctgatctccagtggaaaaaccaccgagcttggagaaatccataacatcatcacag
atgagcagttcactgaaggagtatacaaaattgaatttgcaacaaagactttctggagaaaacttggcctttctccattccatgaatatgttgatgtggt
tttcagtgctaatgacgccgggcatcgccactacaccattgctgttctgctcactccctactccatctcatccactgctgttgtcagtgaaccacacgat
gatctgtaatgacacaaagtcctcttctacagactactaccatttatgtgcagataatacacagtgttttttagctaacattattattttttaatccttg
taaaaccttccagagccccacgctaagttatgttaaccacttgataaataaaccactccttctgaatctaaaaaaaaaaacaaaaggacccccctaattt
ccagaaccggccccccaccttcggctgggacaccttgggactgaggccccaaaacttggtttaacccccccccccctataatgggccctttccaccaata
aaggtcaaatt
3' EST: CF783465 [+]ttttttttttttttcgtttcagaagagtggtttatttatcagtggttaacataacttagaactggactgtagaaggttttacaaagattaaaaaataata
atgttagctaaaaaacactgtgtattatctgcacataaatggtagtagtctgtagaagaggactttgtgtcattacagatcatcgtgtggttcactgaca
acagcagtggatgagatggagtagggagtgagcagaacagcaatggtgtagtggcgatgcccggcgtcattagcactgaaaaccacatcaacatattcat
ggaatggagaaaggccaagttttctccagaaagtctttgttgcaaattcaattttgtatactccttcagtgaactgctcatctgtgatgatgttatggat
ttctccaagctcggtggtttttccactggagatcagctcccagtttccctctgtattcctgaaaacttgcactagcagattggctgcggggatccctctc
actgcatccaatacttttaccataagggggcacttggagtcagcttcaccgtgggaaacatgtcccggtgcagcctccgaggcaattgccagtagggcta
agagaagaaaagacttgaaaaaagccattcttgttcaaagtatgcaattgtgcacaatatgggggggggggggggattaattaactcgagtgcaaaaaaa
aacaattaccttttttttccgggccccggaataaaacccaaaagtgccaattggaacctggggagtgggaccccctggtgtgaaaaaaaaaaggccaaag
ctgggaatttttatttctaataagcggctcccccttaaaaccccctaaaggtggttattgaaaaacaaaatgaaggggttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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