XB-CLONE-4353670
Clone Name: IMAGE:7026260
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Gene:
snrpc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783468 [+]
taattaatcccccccccccccggcagaagagacgtacgcgccattaggatcatgccgaagttttattgcgactactgcgatacgtatctcacccatgatt
cgccttcagtccgtaagactcactgtagtgggaggaagcacaaggagaatgtaaaagattattatcagaagtggatggaagagcaggcacagagcctaat
tgacaaaaccactgctgcatttcaacaaggaaagatacccccaacaccttttgcggctccgccagctggaagtgccatgatcccaccacctccaagcatg
ggtgggcctcctcgacctggaatgatgcctgctccccccatggctggccctcctatgatgccgatgatgggaccacctcctccaggaatgatgccagtgg
gccatggtcctggtatgagaccacccatgggagctcacatgcctatgatgcctggcccacccatgatgcgtccaccgacacgccctatgatgctacagtc
ccggcctggcatggcacgccctgaccgatagaatttttacagttatttgtagctgtgctaaactttgtggtatggatgcttattgcagttcatgcaaatc
aagaaaatcacagcactcgtacaagtgtgcctgctctgtattgccaaaaattggtgcaaaacccatcgatgttaattctctaatataatttgctctgaat
ttaaagggtatcaaacatgcttatatatccagtattccaacttactggcggtaccgaaaagttgcaaagggcatttgttttaacttggttttcggaaatg
gtgaggtcaggtcctggacggtgtccctatgtggttggccttggagccccccaggaaggcgaaaattttttttttggttgggaattttgtgacctagaaa
aaaaggaaaacgtgtttttttacccaaaaaaat
3' EST: CF783467 [+]tttttttttttttttcgttttttttttttttcagttacaacagcaactaaaaatctttaattgataactattttactacaattgtcaaaagtagaattaa
aaacattttaaacagatgtaaatatgcaatgccatattccagtcaaagctagagttgtgggttataaaacatttttatacgcctattccagcaaaattct
ggaacatgacaatgggcaaaatgtgaaaactgtagtaatgtacttttgtccctgcaaaaaaaatactataaaaaaacaatacttctacaataatactttt
acatagtttggtgacagaaattcttatatacaaaaagaagtcgtatgttttttggtgataaaacagtttacatttctctaggtacaaaatcacaacaaaa
aaaaatttctgcatcttgtgtgctcatggcaaacacataggacactgtcaagacttgacatcacatttccgaaatcaagtttaaacaaatgcactttgca
cttttccgtacctgcagtaagttggaatactggaatatataagcatgtttgataccctttaaattcagagcaaattatattagagaattaacatcgatgg
gttttgcaccaatttttggcaatacagagcaggcacacttgtacgagtgctgtgattttcttgatttgcatgaactgcaataagcatccataccacaaag
tttagcacagctacaaataactgtaaaaattctatcggtcagggcgtgccatggccaggcccgggactgtagcatcataagggccgtgtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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