XB-CLONE-4353667
Clone Name: IMAGE:7026259
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Gene:
rpsa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783470 [+]
taattaatcccccccccccccgctcttttactccagctatagcaggaggatctccacacggctttgtctcccgttccccatcgtagctttaaaggggtaa
ctcacaatgtccggaggtcttgatgtcctgcagatgaaggaggaagatgtcctcaagttcctggcagcagggacccacttggggggcaccaatcttgact
tccagatggagcagtatatctataaaaggaagagtgatggaatctacataattaacctgaaacgcacctgggaaaagcttttgcttgcagcacgtgccat
tgtagctattgagaatcctgctgatgtgtgtgtcatttcttcaaggaacactggccagcgtgctgtactgaagtttgcttctgccagtggtgctacgcct
attgctggtcgcttcacccctggtaccttcaccaaccagatccaggctgcttttcgggaacctcggttgctagtggtgacagatccccgggcagatcacc
aaccccttactgaggcttcatatgtcaacattcccaccattgccttgtgtaatacagattcaccactgcgctatgtggatattgctatcccatgtaataa
caagggtgctcattcctgttggtcttatggtggtgggatgtttggctccgtgagggtttctgcacctggagagggcacaaatcttctccgcgaagcaccc
ccctgggaaaggttaagcccccggattttggtaacttcttcccagttgaattccccccaataaaattttttatcacagcgccaccaactcaggggccaca
ccttttcccacgaaaaaaacaggtgcctaca
3' EST: CF783469 [+]tttttttttttttttcttttttttttttttttttgttgaaaagtgaaaatgaaattttatttcaccgtcttttcatgaccttacacacttgggacatatg
tgttcttaagaccactcagtagtggttccagtccattctgatgcttgagcagtaggggcagcagaccagtcatcagtactagctggctgtgaactccagt
cttcagctgcaggttttgctgctggaatttctggacgttcagctggaaactgctggatgggaacagatgggacctgtacaccttcagaccagtcagcaac
ctcagcctgagggaactctgccacaggtgcagtccattctccttggtattcttctttggtggtagccttctctgcagctgcctgttcctccttctcaatt
tcttctggatcacggtagaagtacagatcgggcatgacctcccaggggtgctcacgagagattgtgcctctcatgcgcagaacctcacgagccaacatcc
accacataagaccaacagaatgagcacccttgttattacatgggatagcaatatccacatagcgcagtggtgaatctgtattacacaaggcaatggtggg
aatgttgacatatgaagcctcagtaaagggttggtgatctgcccggggatctgtcaccactagcaaccgaagttcccgaaaaacacccctgcatcttgtt
gggtgaaggtaccaaggggttgaaaccgaaccgccaatagggccgtaaccccactggggcaaaaagcaaactttcagttaaagcccccgcttgggccatt
tgtttccctttgaaagaaatgtgcccccccccccttccggcccaaggaatttctctcaaataagcgcttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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