XB-CLONE-4353652
Clone Name: IMAGE:7026252
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783479 [+]
taattaatcccccccccccaacccccccgctcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaa
ggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaag
agaaccatcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtg
gtatcaccatcgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgat
cactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaatggacaaactcgtgagcat
gccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaa
tcgttaaggaagtcagcacatacatcaagaagattggttacaaaccctgatactgttgcctttgtacctatttctggatggaacggtgacaacatgcttg
agcccagcgccaatatgccttggttttaggggggggaaaatctccccgtaaagagggatctggggagcggaaactaccctgcctggaagctccttgaact
gcatttttggcaaccaagttccccccaactgaaataacccccctgt
3' EST: CF783478 [+]tttttttttttttttatcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaac
actaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgact
attaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgangtgagagatgctggaannatatcattttgcc
tttctgtgcttttctgagcagaactttgtgacttttgccanngctcagcagcctttcttaatcgacggccttgatgactccctaaagcaacagtctggcc
tcatggtcacgggaccgcaaaaacgacccaaaagggggggtaattccaaaaaaactcttccaggcccataaggccttttcctggggatcatgtccaacca
ttggggagcctttcccccaaaattttggggaatttggggaattggtctttccagattttcttaaccaaaaaagggggaaataaaattttttttcttttaa
ggttaaaaaaaaattttttaaaccaaaattggaacccttggtggggcaaaccccaacccagggggggggaatttcccggaacccaatttgggggcgtggg
gggggtttcccggaaaaaaaaaaccccggtttctttaaaaagagacacacccccctccccttggggggggggggaattatttttggggtgccccccccga
ccaaatttttccccccaaggaggaaagcttctctcttttaaaaaaaaaaaattttttttttcccgttaaaaaacacccccct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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