XB-CLONE-4353649
Clone Name: IMAGE:7026251
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Gene:
atp1b2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783481
[+]
taattaatcccccccccccccaggagcttcatgtgagagacaccaagagactagcaggggaaacagccaattgccccaactctcacaccccacagtgctt
taaagcctactgcaccagggcatttattgttggcaaggggcaataagcaagatggcggctatgagtcagaatagaagctgtggggagataatgaacgaat
ttaaggaatttatgtggaacccaaggacccgtggaattcatgggccggacagcgagcagttggggtctgattgtctctttcgacctggtttcccatgcct
tccacacaggtacgtttgccctccccatgctcgtcatgttatagaccatctacgaatatatccccaagtattgtgacctacttactatcccatcactgat
gattctacccccaacacacactcggtaacacgctttacatgagttaccaggcaaagctctttgtccaggcgcctcaataccacccccgcggtagacctga
atagatagggcgccccaaatatttgcaccccctctccccccatatccaaataagccccccctcgtaaatattaccccaaaacgcgaaaagattttcacac
ctcgcctcggtgaatcgtattaatacggcgggggacatttacgattggcatccactatacccccctccataaaatacagagcgctctatgtgatcacaca
ccacacgcactctttttacgcgattgtgtttattaacccgtggggcggtcccaaaagtgatgggtgattttttcgcacgcccccttccactctcgtataa
ccacccccccctcgataanatacatagtaaacagtaaccttatcctattggtgtgagagggatagacatatctaccgcgatgtgtcctacacccgcgctt
agcatcctcttttaacgtgtaaaccgtccttactgacacagtatttagtgattgtatatatctngccncatnagcatccctcgcccgtaagtgnttgctt
ataacttctccccacatcaccacatttctgtcntatatacgttcacgcgcctatctggtttccacacatcactatgccaataacgtgcgcggatgaaatt
gagcagtctcccacgcctcatatgacgcgttatgcctactttgcgcccactcacaccacacatcttacttctgtcgcaccacattttcacacccacatac
agagtgatgtcttgcg
3' EST: CF783480
[+]tttttttttttttttctttttttttttttgggtttgtcgctttattgttgcaccagggggtatgttacaactggttctatttacacccaaagggactgta
caggattcatgaagggttaaatatttcttatttacaatgcaattggttagaaatgcggaatttaaaaataaaatcatatttcttgcagaaccccccccca
cgccccattacttttgctttatttcggttgctaggcaactgaaaagatggtctgtgaatgatacttgagggaggcacccttgcctggcattatgggatat
gttccaggctatgggaacttggcggggcccctggggccccatgagacgccattacggattggtcatttgctttcatttattgatgtgcagcttgaatgag
acgcgccccgaaaacttgtcttgactgtcattggtgttgatattgacggcgttggcgcggcactgcacgtacaggtcttggttcagcgtcaagttgtaga
acttcaccgccaccagcggctgcgaatagttcttctgtgctctgttcccatagtatggaaaatacatgaggtccatggtgcccagactggtgccgttact
ggtgccgttactggggttgctactgggggtataagtgcgactccccaacatctgatccatgtttgccgtcgtgcccgtgcagtttatggtgatggacgta
ttcgagagacttgggatgaccccagggtaaaagtttatgatcttgttcatcttgatgaagagaaaagggcttgccattggaataacccttacgtgggggt
ctgttagcccccaaacaatccccccatggaacttccgagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: