XB-CLONE-4353646
Clone Name: IMAGE:7026249
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Gene:
btf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783483
[+]
taattaatcccccccccccccgatcttgctccccgcgtgtgtgcgcgcccgtcccccggctccgtaggaagcctttaaacccgctctgctcgccaggcgt
gcccgctgtctgccttcagaagatgaaagagacaatcatgaatcaagaaaagttagccaaactgcaagcacaagtgcgcattggtggaaaggggacagcc
cgcacaaagaataaggttgtgcacagaacagcaactgccgatgataagaaactgcagttctctctgaaaaaacttggagtgaataacatctctggcatac
aggaggtgaatatgtttaccacccaaggaacagttattcgctttaacaacccctaagtccaggcttccttggctggcaacacatttaccattacttgggc
atgctgagaccacgcagcttacgggagacgctgccctgtatttcaaatcagtttcgcgacctcatatgccctagcagcttgcgcgaaacttgcataaggc
ccttgtctaaaaacattctgggggttggggaacccccccccctatttttctttggggccggaaacaaggatgcatcaaagttttcatgcaacctggcttt
taattaccctttgtaatgtacgtctgccccagagaacaggaaatccggttccctcgcttttaaccaggctccccattagggttttccccccaccaccttt
tacaaccgccgtgaacatagagagccccctaacgtggtgaagtatttcccaatctgacccactgtcccttttcgtaaacggcggggcacctattcttgga
aacagccttccgcgcccagagacctttatggccctttccaataatagctccccctcctgagattatacgacatatgcaatcctctatataaagccatggc
ccctattacgcgtttacgttttgcccaccccagcacacagacatgtncatgccgtctactccgtggctgccagattatatc
3' EST: CF783482
[+]tttttttttttttttaagtttattctttattcatttacattttaatttccaggactgttcactttttgtggctgcaaaggcaaattatctgatgaatgca
gttactgcagagctgctatagaaagaatttcaaatctgtggggcttttaatctcaaaggtcaaaagaaaaaaacaaaaaaccacacacaaaggcaatgca
gtcacaatataaaataggagctccgtcagcttttcaagttttgttccttttagatatttttcattcagaccgattcattctttgaagcctcatcaaagtt
ctcaaccagttctggtacttcatcatcttcttcctccccagaagctagtggtgctttcccatccacagattgtttaggcaaggcctctgcaagtctccgc
aagctggtaaggctgtctgctccaagctgatttaaaatactgggcagcatctccgtaagctgcttggtctcagcatgcccagtaatggtaaaatgtgttg
ccagccaaggaagcctggactttggggttgttaaagtgaataactgttccttggtttgtaaacatattcacctcctctatgccagagatgttattcactc
caagttttttcagagagaactgcagtttcttatcatcggcagttgctgttctgtgcacaaccttcttctttctgcgggctgtccccttntcaccaatgcg
cacttgtgcttgcagtttggctaacttttcttgattcatgattgtctctttcatcttctgaaggcagacagcgggcacgcctggcgagcagaagcgggtt
taaaagctttcctacgggagcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: