XB-CLONE-4353634
Clone Name: IMAGE:7026244
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783490
[+]
taattaatccccccccccccctctcattcagccggtggcagcggacttgacagctacttcccgctctttgtaacaacttgccagctttgcatttctcctc
ttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgctggag
atgatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccagaaggacagctatgtaggtgatga
agcccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatggagaagatttggcatcacaccttc
tacaatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacagagaaaagatgacacagatcatgt
ttgagactttcaacaccccagctatgtatgtcgccattcaagctgtgctgtctctgtatgcctctggtcgtaccaatggtatcgtgatggactctggaga
tggtgtcacccacacggaacccattttatgaaggttatgcccttggccccatgctaaccctgcgtctgggacttggccgggtccagaacctgaaaaaact
accctctgtaaaaacccgggaccgaaaagaaggttttcagtctttcccccacccaaatacctggaagaaaaaaaaaatattggggcggttggaacatctc
caagggggaggaaaaaaactccggccaatagttgttttgccttccttgtgagaaaatttttc
3' EST: CF783489
[+]tttttttttttttttatttactgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaattttggtt
ttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaacttt
cttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaaa
gatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcagataccctg
tacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctttcacagaa
atgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgctagcattga
tgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatccccccaacagtgactatggtgtgtcttcaaattcctaagaataaagttc
attttggaaagttgggcccttttttcccagttttcaagaaggctagccatgaaggccgtaacaccctttggcccaagggcccaaatttaaccaccttttg
gaaagaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: