XB-CLONE-4353626
Clone Name: IMAGE:7026241
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783494
[+]
taattaatccccccccccccccgccttttctttccccaccctccgccggcactgatcctccctgtgtgctccggcactacaacacccaggccgccaacat
gatcatctacaaggacaccgtcactgaggatgaaatgttctccgatatctacaagatcgccgaaacccccgacggcatgtgttttgaagtggaagggaag
atcatccaaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctgagtgccaggaggaggacatcggtggtgcaacaacagtca
gcggggtggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactcttacaagaattacattaaggattatgtaaaactagtcaaggc
caaacttgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagatagagtaaaactcatacttggaaacttcaaaaactatcagttt
tacactggagaaagaatgaacccagatggtatggtggctctgctagattaccgtgaggatggtgtaacaccttttatgattttctttaaggatggcttga
tcagtgagaaatgttaacattcaatttggttcttccatcttggctgctgcttttcatccatgaacaccaggtctaaaccagatcttactgactcctaaag
gactgatgatacatttgagcttgttttctttcatggccctaaaatttattcaagttggaggcctttttttggtttttttaatttggaaataccttttggt
ccattttagaagtaaaataaaaaagccccattt
3' EST: CF783495
[+]tttttttttttttttcattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgttaacaggcaac
agtcaatgttccaaatccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccagaggtatttgcaacactgttgaaaggcctt
ctggcagtggaggagtctaaaaacctgaagccgagaggcagattgaatactttggctgtttccttaagtgtgcagattgtccacaaactgaaggtccgct
tcttacaaaatgttctggcagtcagtctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaattgcagatcaa
accaagaggcaatgtctacagtggagaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaacaaaaaagcctccacttgaata
atttagggccatgaagaaacaagctcaatgtatcatcagtcctttaggagtcagtaagatcttgcttagacctggtgttcatggatgaaaagcagcagcc
aagatggaagaaccaaattgaatgttaacatttctcactgatcaagccacccttaaagaaaatcataaaaaggggttacaccatccctcacggtaatcta
gccagaaccacccataccatctggggttcattcctttccccagtggtaaaaactgaaaagttttttgaaagtttcccaaggttaagaatttttaactccc
actcttggaaaattccctttccaaaggaaaggggttttctacctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: