XB-CLONE-4353618
Clone Name: IMAGE:7026238
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Gene:
rps3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783500
[+]
taattaatcccccccccccccgttttctctcccagctctgcaagatggcagtcggcaagaacaagaggctgactaaaggtggcaagaagggtgccaaaaa
gaagattgtggaccccttctccaaaaaagactggtatgatgtcaaggcaccagcaatgttcaatatccgcaaccttggaaagactcttgtcaccagaacg
caaggaacaaaaattgcctctgatgggctgaagggtcgcgtctttgaagtaagcttggctgatctgcaaaatgacgaagtggctttccgtaaattcaagc
ttatcacagaagacgttcagggcaagaactgcctgaccaacttccatggcatggatctcactcgtgataagatgtgctccatggtcaagaaatggcagac
catgattgaagcacatgttgatgtgaagaccacagatggctacttgctccgcctgttctgtgttggattcactaaaaagcgcaacaatcaaatcagaaag
acttcctatgctcaacaccagcaggtccgtcaaattcgcaagaagatgatggaaattatgacccgtgaagtgcaaacaaacgacctttaaagaggtttgt
tataaacttgattccagacagcataggcaaagatattgaaaaagccctgccagtcaattttatcctctcccacgaattttttatgttccctaaagtggaa
gatggctgaaaaaagccaaagtttttgaacctggggccaaactaaatggaaactttcattggcccaagggttgggaaggaaccttggaaaaacaccagcc
acggaaaaaccgagaaacttgggtgggccaaaaaatgtggaaacacttgccctgaatatggggtaattgaaaacccccccccggggggt
3' EST: CF783499
[+]ttttttttttttttaggtttattttacagtttttaaatcagaattctttaactcagaccgattcctgcactgggggttcatacccatcagcacgttccac
tttggcaccagtctcgtctcctgctggttttccagctcctccaccctcgccatgaagttccattagtttgcccagctcaaactttggcttcttcagcatc
ttcactttacggacataaacatcgtggagaggataaattgactggcaggccttttcaatatctttgcctatgctgtctggaatcagtttattaacaacct
ctttaaggtcgtttgtttgcacttcacgggtcataatttccatcatcttcttgcgaatttgacggacctgctggtgttgagcataggaagtctttctgat
ttgattgttgcgctttttagtgaatccaacacagaacaggcggagcaagtagccatctgtggtcttcacatcaacatgtgcttcaatcatggtctgccat
ttcttgaccatggagcacatcttatcacgagtgagatccatgccatggaagttggtcaggcagttcttgccctgaacgtcttctgtgataagcttgaatt
tacggaaagccacttcgtcattttgcagatcagccaagcttacttcaaagacgcgacccttcagcccatcagaagcaatttttgttccttgcgttctggt
ggacaagagtcttttcaaagttgcggatattgaaccattgctggtggcttggacatcatacccagtctttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: