XB-CLONE-4353613
Clone Name: IMAGE:7026236
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Gene:
fbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783503 [+]
taattaatcccccccccccccggctgactgctgacctgtccatctagcagtgtacttacctgtccagagccactaacagcaccaccatgacggaccaggc
ccccttcaacaccgatgttattaccctgactcgcttcgttatggaggagggcaggaaagccaaggggaccggagaattaactcaactccttaactcgatg
tgcacggctataaaagcaatctcttccgcagtgcgcaaagctggcctggccaacctctatggtattgccggatccactaatgtgacaggggaccaagtga
aaaaactagatgtcctctccaatgaccttgttatcaacatgctcaagtcctcatacagtgcctgtgtcctagtgtcagaagaagaccagcatgctctgat
tgtggagcctgaaaaacggggtaaatacgtggtgtgctttgatcctttggatggctcttcgaacattgactgcctggcttctattggtacaatattttcc
atctacaaaaagactacagaaggagagccaactgaaaaagatgctctacaaccaggacgaaacattgttgctgcaggctatgccctatatggcagtgcaa
caatgttggttcttgccacagaatgtggtgtcaactgtttcatgttggacccagcaattggagagttcattttagtaaaccaggatgtgaaaatcaaaaa
gaagggcaacatttacagccctgaatgagggttatgccaagtattcctgaatgaagcagtttactggaatacttacagaaaaaaagaaattttccacgaa
gaatggggaagctcaacccctacggggc
3' EST: CF783502 [+]tttttttttttttttcaataatataaaaatctatttgttattgtgtacaaaagtaaacatatttctttatgttttggcacattaaaaagaagctgctggt
ttattctttggtccttcttgatatgaaatccattcagctgtcctttatttctttgcatatttcttgtaaatgttaatatattcctgcacatcatctgggg
agcccaaagctactggcatcctctggtggatactctcaggtactacatcaagaactgactcaagtccattagtagccattcctccggccttctccataat
gaaagccattgggttgcactcatacagaagcctcagcttgccctttgggctctttacattggctgggtatagaaagattcctccataaaccaatgtacga
tgcacatcagctaccattgaaccaacataccttgcaccgtagggtgagctcccatcttctggaaatttctttttctgtaagtattcagtaactgcttcat
cagaatacttggcataaccctcattcaggctgtaaatgttgcccttctttttgattttcacatcctggtttactaaaatgaactctccaattgctgggtc
caacatgaaacagttgacaccacattctgtggcaagaaccaacattgttgcactgccatatagggcatagcctgcagcaacaatgtttcgtcctggttgt
agagcatctttttcagttggctctccttctgtagtcttttttgtagatggaaaatattgtaccaatagaagccaggcagtcaatgtttcgaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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