XB-CLONE-4353610
Clone Name: IMAGE:7026235
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Gene:
snu13
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783504 [+]
taattaatcccccccccccccgaggtccattcgatcatcctccgtgaggcttgcgggctgagaggagaaactttcatcaccgatccactgaacggataag
caccaagcgctgccatgactgaaccagaagtgaaccctaaagcctacccacttgcagatgcccagctcacaaaaacccttttggatcttgttcagcaagc
cgctaattacaagcagcttcgcaagggtgctaatgaagctacaaaaaccctaaacagaggcatagctgagtttatagtcatggcggctgatgcagaaccc
ttggaaattattctacacctcccactcctgtgtgaagacaagaatgtcccctatgtgtttgtgcgctccaagcaagcacttggaagagcttgtggagtct
ctaggccagtgattgcttgtgcagtcaccataaaggaaggttcccagctgaagcctcaaatccagtctcttcagcagtctattgagaggctgctggtgta
atttgatggttgtatgttcaccttcccctttcagaagagctgtaaggcgtttcatcacactgtttgatttagtagctttcaaagaaaaccagtggtgagt
ttgtatctttggctggagccaagttacaacagcacaactgagtgaagtcttatgagttaacacatttaacagcaattgtagctgaagactatattgagct
gtcatcagtttctttttactttttacatttgcaggtgtttttgaattgcttttgtgtcaaacaggatcttgagaagatttattttcattacactgacctg
gtatta
3' EST: CF783505 [+]tttttttttttttttcacatgtatatttttattaaaaatacatacaatacaagtcagtgtaatgaaaataaatctttcaagatcctgtttgacacaaaag
caattcaaaacaactgcaaatgtaaaaagtaaaaagaaactgatgacagctcaatatagtcctcagctacaattgctgttaaatgtgttaactcataaga
cttcactcagttgtgctgttgtaacttggctccagccaaagatacaaactcaccactggttttctttgaaagctactaaatcaaacagtgtgatgaaacg
ccttacagctcttctgaaaggggaaggtgaacatacaaccatcaaattacaccagcagcctctcaatagactgctgaagagactggatttgaggcttcag
ctgggaaccttcctttatggtgactgcacaagcaatcactggcctagagactccacaagctcttccaagtgcttgcttggagcgcacaaacacatagggg
acattcttgtcttcacacaggagtgggaggtgtagaataatttccaagggttctgcatcagccgccatgactataaactcagctatgcctctgtttaggg
tttttgtagcttcattagcacccttgcgaagctgcttgtaattagcggcttgctgaacaagatccaaaagggtttttgtgagctgggcatctgcaagtgg
gtaggctttagggttcacttctggttcagtcatggcaacgcttggtgcttatccgttcagtggatcgggtgatgaaaagtttctcctctcagcccgccaa
gcctcacggaagaatgatca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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