XB-CLONE-4353598
Clone Name: IMAGE:7026231
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783512
[+]
taattaatcccccccccccccgtgtgccaatgactcttcacctgttaggacccgaaacagaattgttaagagtggccacacagagtgagagattttaaac
catggccgacgtaagccagcaaactgaaaccactaccccgtctgttgcccaaaagaagcttcagagagcaatttggttcttccgcacaggagacctcact
acctggaactatgctattctgggcctagcacttgctggacttctcttgggaatgttccttttgatacgaaatattctaggtaacaggaagaggaagatga
ttgaaatgtataagaaaaaagaggcttctgctgtgccagaggaggctgagaacaaacaggcagaggtctacttggaaaaagaagactcttccactaaagc
tactctgttgcagaagggaatgaagcctggggatatcacagtgcaatggaaggacgggcacattgagtccctctatactgcactgccagaagaggatgta
taaaagctccctagtgtattatattaaaggacagcatgtacccaggtctcaagaaaaaaaaataatccacattgcaaagacatttctcatggtcataacc
cttaatacacccattttcctttatggatctccaagaaatgcagggaaatcatgcacagatataattagggtattacaagtctctacctcggtggttgtca
aactttttcagttcaagcttcacttgaaaggtcaaacctttggctaggggtttcctttgtatcttcttagaaaaaaaacagggccggcacaaaaaccccg
gagtgggagtc
3' EST: CF783513
[+]tttttttttttttttcttttttacttttaagatacagaattttatgaacaccacatctcttatgtaacagaaaacggcagcgctatttaggcatgtataa
agtattacaggtaggggtcctgttatccagaatgctcagggcctggggttttgtggaaaagggatttttttgtaatttggatctccctatttctactaaa
aagaaattattttaatagtaattaaacccgaatattaattgttttgcctccaataaggattcattatatcttaatttggatcaagtacaaggtactgttt
tattattacagagaaaaggaattaattaaaaaaaatgaattatttgtttataattgaaccaataggagacagtctatctaaaattctgagctttctggat
aatgggtttctggataatggatcctatacctttataagtaactaaattatccagaagtaatattacaacatccatttaactattttatgcaacccacagt
ctcactgaaaacatgggtttattttaaattaaaattatatagctagacttanaacataaaggccataggcagtccacaggtacaatatctgacatcccct
gaacaattcatggtaaacatcatttcattgcactgtactgttgtctggcacacaatatcctacattaactgtttttgcttgcaaccagccatccaagagt
gcagggtactgtcatctgaaaactttttagtactcacatactttgtagaatccctaataaccttggtaccccctagaaacatcctcaagttccagcttgg
aaaggtctccttagggtggagaattc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: