XB-CLONE-4353589
Clone Name: IMAGE:7026228
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783519 [+]
taattaatccccccccccccccggccactgagctcaaggcaagctgccgaacacttcccactgcaaaactacttactaacaacaaaatttcaataagtaa
gaacctacgtatttctccaggagctccaagaaggggcaaaaatcacatggtggctcaagggtgaaaaacagaagaaaagccaaagcagaactacactacg
agtaggccaaagtatcacatcagacatggatcttgccaataacagaggcctcaacctgatgctaaatttcctgctaattgtgatggtgctactgctaatg
ttcatcatagtaaagctcctgtgagatccttccttcatatcattctgggtaaaagcaacaactccagagataataatgacaattggaaagaactgcaatt
cctttatccctcatgggacataaatactacattttccattctgaaaacaacttgcttttttcacacactaacttaagagagatgtacataattttaagtg
attttaaagaggaaaatgggtgccacaaagcaattagttacctttaacacatttgctggactgcttggagcactgtatatttaaaattattttataatca
agttttatacttgtgtgtgtgtgtgtgtgtgtatatatatatatatataaaatatactgacaggcactctcgacaaacaaagatcaaagatgcctgggtg
cagtacaaaaacggagtaaagtaacagaaaggtactgggcaccacactccacgggattaatgaaaattacaaggaggtttatttcagaggttgtgtctta
acggttttcggctaaccacttcagcct
3' EST: CF783518 [+]tttttttttttttttctttttacagtgggtaaatacaaaacatttttatttacaggcacagtgtatttgcagaacagaatatctgctggtctatctgttt
atctgtcccagtctctgtatcactccatctgtagccatacttgatttttcttggccacctcaaatgtacagtcatttttttaaattttaaaatgtctgat
gtttagttaaaatgccaaggataatctacagctgcctttgctgggcatatagatacaatggacaatccttcttgggtccaataaataatgcaataactgt
gtgttcattcttttacataaaactgaatgatactttaaaaaatacattgtgtaaaagtgcttaaagactcatcttacaggtaagttggtgcgaggtcaaa
gtgttagtggtatgcaacagagctggtgtacaacatcaaaacttaacattaaattcattacttaaatatgtcttgaaagatttctatttaggacagtgag
acttggaatgtgttccagacttccctgccttttttttgtcttcaccctacattttacctgtcaacattaaaatatcattaccggtagtattaaactaaag
gggaatcttttgccatttgtatccatgctgccagggctatttcacaggagaacaatatgccagatacaggttttttagatgtctgtgcttaatggaatat
tctacaacttactaccaattatacaggaacattagtgacttttaatattgttgtaatttccatggcctgtataaaagcaattagcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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