XB-CLONE-4353586
Clone Name: IMAGE:7026227
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Gene:
sec23b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783520 [+]
taattaatcccccccccccccggaagcgctacccggataccggagagcaactagcagggagccagagatacggccagccggtgatagcggtgattgagct
aaagctatctggtttctatggcaacctacttagagtttattcaacaaaatgaggagcgggatggcgttcgcttcagctggaatgtgtggccctccagccg
actggaagccacacggatggttgtacctttgggctgcctttttactcctctgaaggaacgtccagatctcccaccagttcaatatgagccagtgctttgc
acaaggccaacctgcaaggcagtacttaatccactttgtcaggtggactacagagctaaactgtgggcatgtaatttctgcttccaaagaaaccagttcc
ctccaacatatgcagggatatcagaagtaaatcaacctgcggaattaatgccacagttttccacaattgaatacatagtgcagcgaggtcctcagacacc
actggtattcttatatgtggtggatacatgtcttgaggaggaagacctgcaagctctcaaggagtccttacagatgtctctcagcctgctgcccccagat
gctctggttggtctgatcacctttggcagaatggtacaagtgcatgaactgagctgcgatgggatctctaagagctatgtttttagaggcacaaaagatc
tgactgctaaacaaatacaggatatggttgggacttttcaagggctgcagcccccgggcacagcaaaggaagacccactacaaaccccagggaacagcct
tttttttgtcctagtcgggttccttgc
3' EST: CF783521 [+]atgcacaaattttccactggcttgttcctccaacccccagttcattttcagaaacacaaggacctttcacatttaatgagacacaaggtccaatagtacc
agaaactttcaactctcttgatgtcttgacatctagatttgccccaaatgccattttaaacgatccatttggatcttttgtaaacactctctggaatgtt
tgtttaaaaagggatgtgttaaaggaatctccaatcacaatctctccaccagtaaggtttgaacaacatttcatttccaaaagaccagtctgatccaagg
cacaagcataaatgtcaatgcagtgaccatttgctgcagtgcggtttgccaatgcttcatagtgcttagtagcctttttcatgaaacgtgcattgtcttt
ctcaatatcgtgccaagatctaatgggtgtttttagctcatctccaactaccatgccgggcccttgtgttgggggaccacctgtaaacaacattattctg
gctccagtgtttggaagcgtcccctccaataatccaacagctatagacaaagcaacaccagtagatcgtagaggccttttcccttgaggcacaggccagg
gatcacgttgcaattccccaagcaagtctgttagattcatgtcaattttatgaacaggttgcaagaaccgactagacaaaaaaggctgctccntggggtt
gtagtggtcttccttgctgtgccggggctgcagccttttgaaagtcccacctatcctgtatttggtttagcagtcagatctttttgtgcctcctaaaaac
attaactcttaagaaatccccatcgcagctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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