XB-CLONE-4353574
Clone Name: IMAGE:7026223
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Gene:
rps26
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077656 [+]
CTGTGAGTACGCCTACTAAGAACCCAGGCGAGGCGCAGTAGGAAAGCGCTCAGCCAACAGAGTGGCTCGTCTTATCGTTTTCAGTGTTACGTCATCTCCG
TCGCGCAGGGATTGGGCTTTATAAGGGCAGCGCGGCACCTTCCGGTCCTCTTTTCAGCCCTCGACTCCAAAATGACCAAGAAGCGAAGGAATAACGGACG
CGCCAAGAAGGGCCGTGGTCATGTCCAGCCCATCCGTTGCACAAACTGCGCTCGCTGCGTCCCAAAGGACAAGGCCATCAAGAAGTTTGTCATAAGGAAT
ATTGTGGAAGCTGCAGCTGTCAGGGATATCTCAGAGGCCAGTGTCTTTGATTCATATGCACTTCCCAAGCTGTATGTGAAACTGCATTACTGCGTCAGCT
GTGCCATCCACAGCAAGGTGGTCAGAAACCGCTCCCGTGAAGCCCGTAAGGACCGAACACCACCTCCCAGGTTCAGGCCTGCGGGTGTACCCCAGAGAGC
TCCTCCAAAACCAATGTAAGACAACCGTGGCTAAGATGTATCTTACCAAATAAACAGTTTAAAAACAAAAAAAAGAAAAAAAAAAAAAAA
5' EST: CF783528 [+]taattaatcccccccccccccctgtgagtacgcctactaagaacccaggcgaggcgcagtaggaaagcgctcagccaacagagtggctcgtcttatcgtt
ttcagtgttacgtcatctccgtcgcgcagggattgggctttataagggcagcgcggcaccttccggtcctcttttcagccctcgactccaaaatgaccaa
gaagcgaaggaataacggacgcgccaagaagggccgtggtcatgtccagcccatccgttgcacaaactgcgctcgctgcgtcccaaaggacaaggccatc
aagaagtttgtcataaggaatattgtggaagctgcagctgtcagggatatctcagaggccagtgtctttgattcatatgcacttcccaagctgtatgtga
aactgcattactgcgtcagctgtgccatccacagcaaggtggtcagaaaccgctcccgtgaagcccgtaaggaccgaacaccacctcccaggttcaggcc
tgcgggtgtaccccagagagctcctccaaaaccaatgtaagacaaccgtggctaagatgtatcttaccaaataaacagtttaaaaaccaaaaaaagaaaa
aaaaaaaaaaag
3' EST: CF783529 [+]ttttttttttttttcttttttttgtttttaaactgtttatttggtaagatacatcttagccacggttgtcttacattggttttggaggagctctctgggg
tacacccgcaggcctgaacctgggaggtggtgttcggtccttacgggcttcacgggagcggtttctgaccaccttgctgtggatggcacagctgacgcag
taatgcagtttcacatacagcttgggaagtgcatatgaatcaaagacactggcctctgagatatccctgacagctgcagcttccacaatattccttatga
caaacttcttgatggccttgtcctttgggacgcagcgagcgcagtttgtgcaacggatgggctggacatgaccacggcccttcttggcgcgtccgttatt
ccttcgcttcttggtcattttggagtcgagggctgaaaagaggaccggaaggtgccgcgctgcccttataaagcccaatccctgcgcgacggagatgacg
taacactgaaaacgataagacgagccactctgttggctgagcgctttcctactgcgcctcgcctgggttcttagtaggcgtactcacagggggggggggg
ggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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