XB-CLONE-4353558
Clone Name: IMAGE:7026217
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Gene:
tmem230
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077009
[+]
CCGCTGGTGTTGCTGACGCACAGCCTGCCGGGATAATCCGAGCCCCCGTACCATGGGCGCCGGTATGAGGTTGTAGTTTTTGTGTATCCCATGACAAGCC
ATCTCTGCCATGATGCCGTCACGCAACAACGCAGCATCAGGAATACCAAACAGTAAAGTGAAGTACTCCAAGCTCTCTGATACAGATGATGGATACATTG
ACCTTCAGTTTAAGAAGAGCCCTCCAAAAATCCCATACAAAGCCATTGCTCTGGCAACAGTGCTGTTCTTAATTGGTACTCTACTCATTGTCATTGGGTC
TCTGCTTTTAGCCGGATATATCAGCCCTGGGGGAAATGAACGAGCAATCCCAGTCCTGATTATTGGCATACTGGTTTTCCTGCCTGGCTTTTATCATCTG
CGCATTGCGTACTATGCCTCCAAAGGGTATCGAGGTTACTCCTACGACGATATTCCAGATTTTGATGACTGATAATCCAACGTACATTGTGTGGATTAGG
ACATTGCTGTGCCCTAGTAGGAGTAGACTTACTTTAAGAGAGACCAGTTAATGCAGAGGACACATATTGCATAGGTCTAAGAATTTGTTTGTAAGCAACA
ACATGAGGGTTCCCGGCCCTAGATTTACTCTTAGTTAAAAATCAGAGCAAAATAAACAGGTTGTGGGGCAATTGGCACGAACCAATCAACTGGCAGGAGG
TTTCTGACTTAACATAGTTCTGTATGAAAATACTTGGAAAAGTTTCAGATGAATGAGGGTAAATTGGTGGAAATCATTATTCTGCCATACTAATTACTAT
ATAAATCAATTGCAAATGCAGTTTTTATTACATTACATTTTTAAGGGGAAATAATACCCTGTTTATAATGTTTATTGTATTTTTCCTAAAAAGGACAGGG
AAAGGTATTTGGTGTGCACAGGACATTCCTTATTTGCATGTTAAATTCCCTTTGATTTGTAATATTACTGTACTGTAAAGATGTATAGCCATGCTTGTAG
TATAGCACTTTATAAATAAAAATACAGTATAAGTAAAAAAAAAAAAAAAA
5' EST: CF783539
[+]taattaatcccccccccccccccgctggtgttgctgacgcacagcctgccgggataatccgagcccccgtaccatgggcgccggtatgaggttgtagttt
ttgtgtatcccatgacaagccatctctgccatgatgccgtcacgcaacaacgcagcatcaggaataccaaacagtaaagtgaagtactccaagctctctg
atacagatgatggatacattgaccttcagtttaagaagagccctccaaaaatcccatacaaagccattgctctggcaacagtgctgttcttaattggtac
tctacccattgtcattgggtctctgcttttagcccggatatatcaggccctgggggggaaatgaaactaagcaattccccagtcctgaattaattgggca
tactggatttttccctggcctgggcttttttatccaatctgcccccatttggccggtacctaatgtcccttcaccaaaggcggttattcgaagggcttta
acttccctttaccgaaacgaaaatacttttccaagaaattttttctttaattgtcaactcgggaaataacgccccccaccaccggttaccacatttttgt
ggcgggtgggggcatttttaaaagtgtacctaccttttggccttgtgtatggtttccccgcccaatttgtagagaggaagaggcacacacaatcgtcttc
ttctgttttataaaggaacataaacaaactgactgaggttttataacctccgccccctaatatacacacactccgttaacttatggacgccataagagtg
tcgccacagaaaagatttttcgcgtatgttcgaccatagctcccttaccaataacctgatttcgaacttttctccgcggggcctcccttcgacaattatt
tattaccctctcccgatttaatgatacctaccaaacgtttttcacttttccaatcccccattttgtgttgcgcgctctatcattttcgttttcgcaacac
caccccttctcttctcgcgcgtcgcaccaagggttttctct
3' EST: CF783538
[+]tttttttttttttttacttatactgtatttttatttataaagtgctatactacaagcatggctatacatctttacagtacagtaatattacaaatcaaag
ggaatttaacatgcaaataaggaatgtcctgtgcacaccaaatacctttccctgtcctttttaggaaaaatacaataaacattataaacagggtattatt
tccccttaaaaatgtaatgtaataaaaactgcatttgcaattgatttatatagtaattagtatggcagaataatgatttccaccaatttaccctcattca
tctgaaacttttccaagtattttcatacagaactatgttaagtcagaaacctcctgccagttgattggttcgtgccaattgccccacaacctgtttattt
tgctctgatttttaactaaaagtaaatctagggccgggaaccctcatgttgttgcttacaaacaaattcttagacctatgcaatatgtgtcctctgcatt
aactggtctctcttaaagtaagtctactcctactagggacagcaatgtcctaatccacacaatgtacgttgggattaatcatcatcaaaatccggaaaaa
tcgtcgtaaggataaacctcgaataccctttggggggcataataccccaaggcgcaaaatgataaaaagccaggcaagaaaaacaataatgcccataaat
cacgaatggggattgcccctttcaatttcccccaaggccggaaatatcccggttaaaaagccaaagaccccaatggaaaatggggttagagtttcccaat
tttagaaaacccccctgttttcccccaacacaagggcttttttatggggaaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: