XB-CLONE-4353555
Clone Name: IMAGE:7026216
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Gene:
tmem111
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783541 [+]
taattaatcccccccccccccgagtatccgcctgtgggtcgtcttacccatcgtctgtatcaccttcctcaccggcctcctccgccaccatgttacccgc
ctgctgcagggggagaagcgccccgaactggagcccctcagcgatagccaggtgctgatccggagccgcattcttagggagaacgggaaatacatccccc
gacagtcgttcctcatgaggaagttctatttcaatgaccaagagacgggatttttcaagaaaaccaagaggaaagtgatccagcggaacccaatgaccga
ccccactatgatgaccgacatgatgaaggggaacctcaccaacgttcttcccatgatcctgattggagggtggataaactgggctttctctggcttcctg
accactaaggttccgttccccttgacgctccgattcaagcccatgttacagcgtggcatcgagctggagtcactggacgcatcatgggttagctccgcct
cctggtatttcctcaatgtctttggcctgcgcagtatgtacaccctgctcttggggcaagataacgccgccgaccaaagccggattatgcaggatcagat
gacgggggccgccctctccgtgccgccggacaccaacaaggctttcaaggctgagtgggaagccatggagatctcggaacatcactgggcgctggaggcc
atcgaggaagatctgatcgctcaagatctggaacttgccggggctcaataaaaccgggatccatttgggaatcggtttagtgcccaataaaaagtatttt
ttcctaccagnaaaaaaaggaaataaaaaaaaaaaaggatcccccttagttcctaagaccgggcct
3' EST: CF783540 [+]tttttttttttttttctttttttttttgtaggaaaaatactctttatttggcactaaaccgattcccaatggatcccggttctactgagccccggcaagt
tccagatcctgagcgatcagatcttcctcgatggcctccagcgcccagtgatgttccgagatctccatggcttcccactcagccttgaaagccttgttgg
tgtccggcggcacggagagggcggcccccgtcatctgatcctgcataatccggctttggtcggcggcgttatcttgccccaagagcagggtgtacatact
gcgcaggccaaagacattgaggaaataccaggaggcggagctaacccatgatgcgtccagtgactccagctcgatgccacgctgtaacatgggcttgaat
cggagcgtcaaggggaacggaaccttagtggtcaggaagccagagaaagcccagtttatccaccctccaatcaggatcatgggaagaacgttggtgaggt
tccccttcatcatgtcggtcatcatagtggggtcggtcattgggttccgctggatcactttcctcttggttttcttgaaaaatcccgtctcttggtcatt
gaaatagaacttcctcatgaggaacgactgtcgggggatgtatttcccgttctccccaagaatgcggctccggatcagcacctggctatcgctgaggggc
tcccgttcggggcgcttctccccctgcagcaagggggtaacatggtgggcggaagaagccggttgaagaaaggtgtaaacaaaacgatgggttaaaacga
accc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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