XB-CLONE-4353549
Clone Name: IMAGE:7026214
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Gene:
adss2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783545
[+]
taattaatcccccccccccccacccccccccgctggagagggtaggaggagtaggaagggaaagtggcattttgtatagaagcgtgtcaggtttgagagt
tctacaggaagtagtgttataggttgctctgtacttctcccagcctttcggccatatcctttgtaagcttaacccactgtcagtaatcatgtcggcagaa
aacggatggcctgggctgccaaacggcggagcatgctgcgccactgacagtggacgttgctccctcgtcgggaacaaagtcacagttgtgcttggggctc
agtggggagacgaagggaagggtaaagttgtggatcttctggctcaggatgcggatatcgtgtgccgctgccagggaggaaacaatgcaggccacacggt
tgttgtggattcagttgaatatgattttcaccttttgccaagtggaatctttattcaaaaggctattgcctttctaaagaaatgggggaataattcttct
tcctggcttttttttggaagctgagaaaaatttttcgaaaagggccaagttttggtttggcccgcgcaaaaaaagatttgtgttttcaccggaaaaaaag
ccccaccttggggtgttgaatttcccctccggggctcggcgagtgtgtctatccttggtgagccacacgaaccgtcaagattaacaagcgtggggataaa
gatttgggcgcgcccctcctaataaaatgggcttttgggtacctaattccaatttttatccataggccatggtagggaaatgtgagcctccaaaaagggg
gtgatatcttggtttccttaactttctgtggttgttcttcacccacaaggtgatacaaattatttggtataagnaatgacccaaagcgccgtggtagggg
cgtcaggcacgaagttttcgtctcttgctcggggcgtaagnacgagcgccttctgcgatgtatttctcgccacacattaagactatattg
3' EST: CF783544
[+]tttttttttttttttgttttttttttttttttttagttccttaattatttattttaaatttgaacaatttttatgcaaccatgcttttcttgggttggaa
ccttaaactcttattcaataatttacaaatatagactgtctacccatttgttttgcacagtgcaagtattgcagtgcaatacattttaaaacaaaatcat
tcacaccaattatgattgctttaaaaatatggatatttcctccccatacattacatgagttatgttaagaaatcaatagaaagatgtaaaaatgtgtaaa
agaattctgattcagtctcacaacttgaaattgcagactgtcaacaaagttgtaccagatgcgcacatataggtactagggtgttttttttaattttata
cagaaaaaatagccagttcaatgtgtgatggtcctttccaaaactgaacaaagaagtcactcagtctttaaagtactgccagtttaataaccagaccaca
aatgtcctaatgcgatagaaaataaacagtaggacaattttgactgctttcttttactagattgtgaaaaagactaaataaattagggcataacaaaaga
tgaatctggaagtgatattttgttgcccatgcatttgttctctatgtacaaatgagaagctttatctgtaaaatggggtcatccttgaagaggcaaccct
gtgcactgttgcatctttcttaaaaaaagctgaatccatccgactcttctgggaattttcccaaccacccaatcccacttttaaatagggaaatttccca
aggtttcttaatttctaatac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: