XB-CLONE-4353547
Clone Name: IMAGE:7026213
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Gene:
mrto4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077055
[+]
CGGAGGAGAAGCTTCAACCATGCCCAAGTCTAAGCGGGACAAGAAGGTTTCCTTAACCAAAACAGCCAAGAAAGGGCTGGAGGTGAAGCAGAATCTCATA
GAAGAGCTGCGGAAATGTGTCGACACCTACAAGTACATCTATGTGCTCTCTGTGGAGAACATGAGAAACAACAAACTGAAGGACGTCCGGAACGCATGGA
AGCACAGCAGGTTATTCTTCGGGAAGAACAAGGTGATGATGGTGGCGCTGGGGAAAGGAGTCAGTGATGAATACAAGGATAACTTGCACAAGCTGAGCAA
GTGCCTGAAAGGCGAAGTCGGACTCCTGTTTACCAACCGGACAGAGAAAGAAGTGAAAGAGTGGTTTGATCAGTTCAAGGACATGGATTTTGCCAGAGCT
GGGAACAAAGCGACGTACGCAGTGGTACTGGATGCTGGGCCCCTGGAACAGTTCACTCACTCAATGGAACCGCAGTTAAGACAATTGGGGCTCCCCACTG
CTCTGAAAAAAGGAGTCATCACGTTACTGTCAGACTACGAAGTATGTAAAGAGGGGGACGTATTAACCCCCGAACAAGCCAGGATTCTGAAGTTGTTCAG
CTTCCAGATGGCTGAGTTTAAAGTCTCCATCAAATCCATTTGGACCGCGGAGACGGGCGAGTTCCAGAAGTTTGGGGATGAGACGGAGGAGAAGAGCGAA
GAGGAAGATATGGATGAGGAAGAAGAGAATAACGATGCCTAGGAACACAATCTCCGACCCCCCCAGCCCAGGGGTCCGGTTCTGGTTCAGATTCAGGACA
CTTATGCCGCTCCGGGACTGCTGATACCGACAGCCAATCCAATAACAGAATGTTCCTGTGTTTTAACAGATGACATTTTGCCCTAAAATGCCTTCCAGTG
GGCGTCGCCCTGCAGTGACTGGCGGTTTGTGTGGGTTACGGTCGCGGTGCCCTCGGCCCGTTTGACACCGGGTTTAGCGCTCACTCTGTTCCAAACACAA
TCAGCTGCATTTTAGGAATAAAATCATGTGATTTGGTAACAGAACAAGTTTCAGTTCTTGTGAATTCCCCTCTCTGCTTCTTGTTATTTAAAGGGGATAT
AAACCCTGTCGCTTATTACACACAATATCAATAAAAAATGGAAACCAAAAAAAAAAAAAAAA
5' EST: CF783546
[+]taattaatccccccccccccccggaggagaagcttcaaccatgcccaagtctaagcgggacaagaaggtttccttaaccaaaacagccaagaaagggctg
gaggtgaagcagaatctcatagaagagctgcggaaatgtgtcgacacctacaagtacatctatgtgctctctgtggagaacatgagaaacaacaaactga
aggacgtccggaacgcatggaagcacagcaggttattcttcgggaagaacaaggtgatgatggtggcgctggggaaaggagtcagtgatgaatacaagga
taacttgcacaagctgagcaagtgcctgaaaggcgaagtcggactcctgtttaccaaccggacagagaaagaagtgaaagagtggtttgatcagttcaag
gacatggattttgccagagctgggaacaaagcgacgtacgcagtggtactggatgctgggcccctggaacagttcactcactcaatggaaccgcagttaa
gacaattggggctccccactgctctgaaaaaaggagtcatcacgttactgtcagactacgaagtatgtaaagagggggacgtattaacccccgaacaagc
caggattctgaagttgttcagcttccagatggctgagtttaaagtctccatcaaatccatttggaccgcggagacgggcgagttccagaagtttggggat
gagacggaggaacaaaagcgaagaagaagatatgggatgaggaaagaaaaaaataacgattgcctaggaacacaaatctccgaaccccccccaggcccaa
ggggcccgggtttctgggtttcaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: