XB-CLONE-4353536
Clone Name: IMAGE:7026208
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Gene:
spint2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783553
[+]
taattaatccccccccccccccccagactcagtttgacttgcacagtccgcctacgcggatccctatagtaatggcggcgtggctgggtgggcttctgct
gcttatattccctttggcactggcgcagcagccagcatgtgacggatacgaggtgttggatgggttctgtctgccagaagtgggtacaccggctcggcag
gcgatgaatgagcttgtctcctcagggaacgccagctccgagcaggagtgttgggaactttgctgctccactgagcactgcgacttggctgtgatggaag
agggaagctgcaaactgatcggctgtctgtatagtggcttcaatatgtgtgaactggagagcagggaaggaacagaaagtatcgcagaaaagacattggg
ccaacacccaccctgaaaagatttctgttaccagaagctgtcacagggacatgcagagctgctttttgaaggtggtggtacaacccaaatacccagaact
gcgaaaactttacttatgggggctgcaaaggggacctaaacaatcttatgggcgaagaaggtgggcatgaaaaaaatgggcttggaataaacagctgaaa
atggctaaaaggaaatcctggccccccaattaaaacgtttgggttgaaaaccaatattccaaacccacctttgtacttgggaaaaggggttaataaggga
aattggccaaggtttcctttcccccgggtgggggcctttgaaagccggaatttccaaaaaagggcgaattccttttttaaaaaaaggggagaaatatggc
ccgcccacctaaaa
3' EST: CF783552
[+]tttttttttttttttccagactaacctttattagaagttacaagttgtatatgaaaaaataaaatgtcactccttaaaaacaaacaacccaatttatgat
tcaaaatctcaccaaaatcaagactaataaaaaaaggaaaaggaaaagcatttgggataaacacttttaaagactccacaaatatagtgaaatacagtga
cattccagatgtggcaatgtttccttaccaaagtaactacccacaaggagaactacataatatcagtaattaatttaaagattttgacatctctacataa
taaaatgagagcaaagcaaacagcaactaagtctgtgctgatggacagaaaatatatataaggaaaaaaaaaaaagttgcttttgtaacaaaggcagttt
tcatcactactaggcaagatttaaagggctttcttgctgccagggtagcaacaaaattgcataagtaaaagctacttaaaaaaaaggttgaatttatttc
atgggactcagacttatataggtgaaaatttaaatgtcaaaattacagaagttgtaagactgctgagaatttatcctaggtatccaaaaaaaagctataa
aacatgccacaagtccttcccaactggctgcagtgggaagtgctgaaaaaagcaatccactagttattgctgcctggatgcatgatggtgaggttttttt
aagctcatgccagggaatttcctgttgcattcagtgcaaaacctgcaatatgagttttttgggggaactagtattttaagcaaatagtgaatgaattaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: