XB-CLONE-4353517
Clone Name: IMAGE:7026200
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Gene:
cct5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783565 [+]
taattaatcccccccccccccagttctttcattcgattctttgtcgcagtcatgtctgcgcttggaactctcgccttcgatgaatatgggcggcctttta
ttatcatcaaggaccaggatcgcaagtctcggctcagtgggctagatgcgctgaagtctcatattatggcagctaaagccgttgcaaacacgctaagaac
atcacttgggcccaatggacttgacaagatgatggtggataaggatgggcaagttacagtcaccaatgatggtgctacgattcttagtatgatggatgtg
gatcaccaaattgccaaactaatggttgaattgtctaagtctcaggatgatgaaattggagatggcactacaggtgttgtagttcttgctggtgcattgc
tggaacaagcggaacagttattagatcgtggtatccaccctatcaggattgctgatgggtatgaacaagcagcacgtattgctgttgaacatcttgacaa
gatcagcgacagttttccagttgatccctgagaacttggagccacttattcgaaccgcaatgacaacattggggctcaaaagtgatcaaccgctgccacc
gacagatggcagaaattgcagtgaaaagcatttttgactgttgctgatatggatcgcaaggacgtaaaactttgagttaaaaaaaggttgaaagaaaaat
ttggaggtaaaccttgagggatacccaactggttcaaaaggtgtttataattggacaaaggaactttcaatcccccctccaaaatgccaaaaatttagtg
aaaagatgcctaaaattccgccctttctccaccatgttcccatttttgaaaccaccccaaaggccccaatgcacaaaaacaatctaagcctcaaaaatgg
tttaacctttccagggggggaaaagacatttataaacagggccccttctcccgtaaaaaatttttggaanaaaaagagaaaaagattttttcttctgaga
aaaaaggggggg
3' EST: CF783564 [+]tttttttttttttttaagttaacagcagaatttttattgtcgatgtaaaatatgcagtatatgagaaatgcaaaatttagcaagtagcactgagctgaaa
taagtgttttcctgtttgacggcgatatgaagtttactcttcaatttcaccaggccgacggatgtcatcgattttcaaaatcattttaacaacttgtgta
gccaaagaaatctgctgtttcttgccaattaaagtttctatgacatgctgctgctttatatcatttgtgcccttatgcaggcaatcaatgccaagtgctg
ggttgctttccttcacttgcttagatcgtacttcagtcattgtctgaataggattcataccactgttttcagcaagtgacataggaataatttccaatgc
gtcagcaaaggctctcatggcatactgttccagagatggacacttgtccgcagcctcgctaacggctaaagcacaggagatctctgcagccccacctcca
taaacaattcgattgtcccttacaagattacgaataacacacaaagcatcatggagtgaacgctttgcttcttcaattatcatcttatttcctcctctga
tgaatttagttacagctctagagttcttgcattgctcaataacaagcattcgattccttggttgtgccaaaagaaatttctttcacaataccagcataac
caagtttttctggagttaactcacaaaatcgtgggacaatacgggcctcctgttgcaatagcaattaactctatttcagggtccaccaacccatcgnaac
agcaggaaagttcattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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