XB-CLONE-4353512
Clone Name: IMAGE:7026198
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783568 [+]
taattaatcccccccccccccgaaaaaacctcctctctggctctgagtagagttcttgcttcaacagtgtttgaacggaaccctctctgagtctttttta
gaccaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcgtc
agaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgatcg
tgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaacgt
gggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaagactg
tgaaccaggctttactggatctgcacaagcttggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggaggaacaggtga
aggccatgaaagaacttggagactacatcacaaaccctgaaccgccttggggtgtccccaaaatggattgggcgagtacctgttcgaaaagccaaaccct
gggggaaagtaacttaaaaggcactcttaggggtgggaaacaacaagtccggcttttctggttttcaaaacactggctctcatttaccaaactggggggg
gattaccacaaaaaaagccccccgggccctcagggtggaacaacccttttttacaccctgagcggaatgaacgggggataattaaattcctgtgttgctc
taaataagtgtg
3' EST: CF783567 [+]tttttttttttttttgaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttatt
cccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctga
ctggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgcttgtcgaacaggtactcgcccatgccattctgcggcaccccaaggcgc
ttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgt
cagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacg
ctctggtttcttgatatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctcc
ttgaagaacttggccacatggtggagtgccacgtcatcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcacca
tacggttgatggcggcttcgcagtcgctgttgaagttctgacgcacctgggattgcatggcggcgttttccgtgtttgctgggtggccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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