XB-CLONE-4353483
Clone Name: IMAGE:7026185
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Gene:
ppia
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783584 [+]
taattaatccccccccccccccgcttttccgtggttctcttgggctgtttagagttcagggggttaggaagctgcacgttgcgctcacggcaaggcactg
tttattttcattctgcactatggctttacccagggtctttttcgacatcgccgttgatggctgcccgatgggccgtattatcatggagctcaggagtgat
gtagttccaaagactgcagagaacttccgtgccctttgcacaaacgagaaggggtttggctacaagaactctggcttccacagaattatccctgaattca
tgtgccagggaggtgacttcaccaaccacaatggaactggtgggaaatctatctacggaaacaagtttgctgatgagaacttccagctgaggcacactgg
ccctggaatcctctctatggccaatgctggagcaaatacaaacggctcccagttcttcatctgcactgctaagacttcctggcttgatggaaagcatgtc
gtgttcgggaccgttattgatggcatggatgtcgtgagaaatatggagaaactaggttctcagtctggaaaacccgccaaaaaaaatagttattgctaac
tgtggtcagctttaaacaatgcatcctttagttgttagttcttgtttttttgttttgtttttttccttctggtcttaaacaagcattcctctgncacatt
aactggtagcttcagaaaaccccattcagtacttaaaatcttagtgctctttaaactactgcatttttctgtgggtgcttatcccagttcctatgcccat
ccaaata
3' EST: CF783585 [+]tttttttttttttttattgtgatcatcattttaatctcatcgacacaattttggaatttgtggaaaatttggatggcataggaactggataagcaccaca
gaaaatgcagtagtttaaagagcactaagattttaagtactgaatggggttttctgaagctaccagttaatgttgcagaggaatgcttgttttaagacca
gaaggaaaaaaacaaaacaaaaaaacaagaactaacaactaaaggatgcattgtttaaagctgaccacagttagcaataactattttttttggcgggttt
tccagactgagaacctagtttctccatatttctcacgacatccatgccatcaataacggtcccgaacacgacatgctttccatcaagccaggaagtctta
gcagtgcagatgaagaactgggagccgtttgtatttgctccagcattggccatagagaggattccagggccagtgtgcctcagctggaagttctcatcag
caaacttgtttccgtagatagatttcccaccagttccattgtggttggtgaagtcacctccctggcacatgaattcagggataattctgtggaagccaga
gttcttgtagccaaaccccttctcgtttgtgcaaagggcacggaagttctctgcagtctttggaactacatcactcctgagctccatgataatacggccc
atcgggcagccatcaacggcgatgtcgaaaaaaaccctgggtaaagccatagtgcagaatgaaaataaacagtgccttgccggtgagcgcaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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