XB-CLONE-4353471
Clone Name: IMAGE:7026180
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783592 [+]
taattaatccccccccccccccgtctccccagtcattgccggaagagaacggttgtacctacaagtagaaagtaaaaatgtctatcaagaagatccttgc
acgtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccact
ggcatttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtcggaagagctgtcaaatatataaatgagtttctgggac
ctgcattatgcacccagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgc
taatgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaaccca
gaggtcatccttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtg
cagacacctttaaggaggccatgcgcataggagcagaagtgtatcataacctaaagaatgtcatcaaggctaaatatggacaagatgccactaatgttgg
tgatgaaggaggctttgctcccaatatcttggagaataaagaaggccctggagcttctgaaaactgccatcaaacaaggctgggctacccagaataaagt
tgtaattga
3' EST: CF783591 [+]tttttttttttttttcaccttcaaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacac
aagactacaaagttgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaattttgaataatcctaag
cagcaaagcttttaaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagca
gtgacatgtaagacctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggtttt
ctgaaatttcttccagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcac
ctgtcttgatctgtccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatt
tgactgagccagcttgcacgcctgcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttg
gcaatacngtttagggttggtcactgtcaagtcatcaccaactacctggatattcgaagatgcagtaaacttttttcagggcttcccagtcatcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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