XB-CLONE-4353468
Clone Name: IMAGE:7026178
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Gene:
foxj1.2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081355 [+]
GGGCATTGCGTAGTTATCTGCTCAGGGTTCAGTGTCAATTGATCTCAGGACACGGCTCACAACACATCTCCTTATCAGTCTTGGCTTTTAATTGGGGCGG
AACTCAACAGAAAAAGAAAGTGTGTACCTTCTGCCCCAGAACCATGCCAGTCCTTTCCACCGCTCACCGGCTCCCGCTTTCTGTTGAACGAGACTGCAGT
CGGGAGGATGATAGCCTCACCAACCTCCAGTGGCTGCAGGACTTCTCCATCTTGAGTACAGACTTGTCTTCCATCGCCAACAGTCGCCCCCCTCTTCCCC
GTGCTTCTCAGGGACCTTGCAGCCCACCAGCGGGTGATACTGCTTCTTGCCAGGCTCCTCGCACTGGAAAACAGAGATCAGTCGCAGTGCCCACTGCCTG
GGCCTCCCTGCCAACTCCATCGCCCAGCCCTGTACAGGAGGTGGATTATAGAACCAATGCTAATATCAAGCCACCATATTCCTATGCCACCCTCATCTGC
ATGGCCATGGAAGCCAGCCAGCAGCGTAAACTCACCCTATCCGCCATTTACAGCTGGATCACACAGAACTTCTGCTATTATCGGCATGCTGACCCAAGCT
GGCAGAACTCGATCCGCCACAACCTTTCTCTGAATAAGTGCTTCATGAAAGTGCCGAGAGGCAAAGACGAGCCGGGTAAAGGCGGATTCTGGCAGATGGA
TCCTCGCTATGCTGACATGTTTGTCAATGGGGTGCTGAAGAGGAGAAGAATGCCTGCCTCACACCTTGACCCACCACGCTGCAACAAGGCCATAGCCCAC
CATCCCTATCTGCCAGTTTCAAGACCCAGCAGCCATCACATGCAGCACATATCTGGTGGCCACAGGCAGTCTCGCCGGTATGAGAAGCCCAATCCTGTAT
TGCCGGCGCTTAGAGCTCCAGAACGGCAAGGGGACGCCCTGTTCACTCCTGAAGACCCCCTGCAAGGGAGTAACTTTGATGATCTGGATTTACAAACAGC
CTTAATATCCATGTGCTGGGAAGGAGACCTAGCGGCTTCTAATCTTAATAGCACCCTCACGGGCACCTCAGGGATGGACATGAATCTGCAGGATCCTCCC
ATGCAGGACAACCACTGGTACCTTTCTACTGAGGGGCAGCAGACCTGGGAACAGGTCAAGGAAGAACCCGTGGTGGAGCAATGGTACAACGAAACTGGCT
TTGTGGAAGACGTGCTTTATGAGTGTCCGCCTTGGGAACGGGTGGAGACCTTGTTATGACCTCAGACTTATTTACAGCTTTGTTAGAAGTGGAGGAACTC
GTACTGAAAGGGGAGAGAATCCATCATCAAATCTCTCTGAACTCTTGTTTGAAGAGCTGCCAACCTCCAAACTGCATAAGGAAGAGAGAGATTGTTGCTC
CGGATGGGACTTGTATTTCATTGATGTATGGATCCAATCAACCTTGTGGACTTTGTTGCCATCCATTTCTCTGTGCAGAGAGCCCTTAGCCATATGACAT
CTTTAGAGCTGCTCTGCCACATACATTCTTGGAATATCCGAACCCCTTCCTGTTCTGGGCCTTGGACTTGATTGGAAACTGGTCTCTGCAATGTTAGAGT
GAGGTCTTGAGAAACTGCTGAGAGAAGTCTATTGAAAGAGAATTTAACGTAACTGGCAGAATATATAGATATTTTTTACAGATATTTTCCAATACCTAGG
AGTCTGGGGAATCATTTTCTGATGATAAATGTGCCGCAGTGCCTTCACTTGCAGGATGCTGGGTGCTTTGATTTAAAAGACAAAGGGAATCCCTGCTCCG
GATCAACAGGCCTTCATTATAGGGCACCTGGGCTGTGTATGAAATCAACAGAGACTTTTGGCAGCCATTGTTGTGTTATGAATATTAGGACTGGTAACCT
GTGTGCACACGTTGCCTTAATGTGAGTGGTTATGATACGGGGAATACAAGTTTTCAATTTGAAAAAAAAAAAAAAAA
5' EST: CF783594 [+]taattaatcccccccccccccgggcattgcgtagttatctgctcagggttcagtgtcaattgatctcaggacacggctcacaacacatctccttatcagt
cttggcttttaattggggcggaactcaacagaaaaagaaagtgtgtaccttctgccccagaaccatgccagtcctttccaccgctcaccggctcccgctt
tctgttgaacgagactgcagtcgggaggatgatagcctcaccaacctccagtggctgcaggacttctccatcttgagtacagacttgtcttccatcgcca
acagtcgcccccctcttccccgtgcttctcagggaccttgcagcccaccagcgggtgatactgcttcttgccaggctcctcgcactggaaaacagagatc
agtcgcagtgcccactgcctgggcctccctgccaactccatcgcccagccctgtacaggaggtggattatagaaccaatgctaatatcaagccaccatat
tcctatgccaccctcatctgcatggccatggaagccagccagcagcgtaaactcaccctatccgccatttacagctggatcacacagaacttctgctatt
atcggcatgctgacccaagctggcagaactcgatccgccacaacctttctctgaataagtgcttcatgaaagtgccgagaggcaaaaacgagcccgggta
aaggcggaatctgggcagatggggatcctcgctatgcttgacatgttttgtcaatgggggtgctgaaaaaggaaaaagaatggcctgcctcaaaaccttt
gaaccaaccaacgctggaaaaaaagggcccataaccccaa
3' EST: CF783593 [+]tttttttttttttttcaattgaaaacttgtattccccgtatcataaccactcacattaaggcaacgtgtgcacacaggttaccagtcctaatattcataa
cacaacaatggctgccaaaagtctctgttgatttcatacacagcccaggtgccctataatgaaggcctgttgatccggagcagggattccctttgtcttt
taaatcaaagcacccagcatcctgcaagtgaaggcactgcggcacatttatcatcagaaaatgattccccagactcctaggtattggaaaatatctgtaa
aaaatatctatatattctgccagttacgttaaattctctttcaatagacttctctcagcagtttctcaagacctcactctaacattgcagagaccagttt
ccaatcaagtccaaggcccagaacaggaaggggttcggatattccaagaatgtatgtggcagagcagctctaaagatgtcatatggctaagggctctctg
cacagagaaatggatggcaacaaagtccacaaggttgattggatccatacatcaatgaaatacaagtcccatccggagcaacaatctctctcttccttat
gcagtttggaggttggcagctcttcaaacaagagttcagagagatttgatgatggattctctcccctttcagtacgagttcctccacttctaacaaagct
gtaaataagtctgaagtcataacaaggtctccaccccgttcccaaggcggaccctcataaagcacgtcttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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