XB-CLONE-4353455
Clone Name: IMAGE:7026173
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783601 [+]
taattaatcccccccccccccgagatggacagtaatgtagaaacacacacaaagatagaggagttaggttagggagtagtcctgaaggctgatccctata
gaacagatcctgtgggcatatttaaaggaactctgtatcatttgctgtctctgggctggataatcgaaataaattagcttcatctctggaaaagaaccgt
ggttgtttccttttacctggttgtggtaaatcttggaatatccagtatatttgtaatatactacatctacactatacacaacattttggtgccgtgacta
gtgaccagaagaagcgtggaagatttgtgactcaagagtctgcagtgaagccctgggagggagtgtggaaccctaggacggtggtctgggaatctgtgac
tgtgaacctaaagggcattttctacatcactgactacttgctgcccccctcaccctaagtctaccttccattgagatgaaacctgcccgaaccaaacaaa
aaggggaaggatcctccaaacgaacctccaagtggtctccccggaagaatccggaatcctgcatgggcatgtcccagaaatgccccccccccgaacaaaa
gggttttgcccagcctttgcccaagaaactggcaccaagggggggcgaacccccttaaccttccccaaaaaaatcatatttcccctaattcaagggaaaa
acaggttttcccgaggggaatctccaagcctctttagcaaaacctccccggggaaaaaaaaacccccctacggggggagggaactcccccccaagtgggg
accaaaagggggacaaccataaacgggggaaatttctccaaataataacccccttataggggaaggggtggtgg
3' EST: CF783602 [+]tttttttttttttttgccagaagaattattaatttttatagtgcatgtgatgtggagtacataaatccaggtaaaagagatgaggtcagacttaggttgg
aagctgacatggaaatcagacaattaattttatcagtattcccaccacaggtaacaacatttatgaaccttatggtaaaaccagacatggactatgagaa
atggattgggtttttacaattagagtggaataaaatgcaagtttgcactccacttaatgagttgcatggggatataaagaatgccagaaataactggagg
agccatacacccaagcaatttagggttaggagcaacagaagaggctctgggaaaaatgggaagtggattccattccaccaaataaaacaagaaaaggaaa
tgctggaagataaagtaaaagtattagaaaatctactaaaacagcatggagtgcctctcccctatactcagtaaacgcaccaagcgctctgtttttgccc
cttcttcagctagtactttactgtttacaattttttaaaaatattttagttaggttatttatgaaataaagtatttttttttcttttctttcttttctat
tagaaaggcatgaatattttaaaatggcaatggattttttttttctccttcaacacatttgttaaaaagtgttgaattgttgagtagttgctgcagtgat
cgcaactgggtttaaagaaacaaaaatacaaatacaatattttttttttttaaaaaaaagttttgctgtgatacactagagacaaaagcat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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