XB-CLONE-4353436
Clone Name: IMAGE:7026166
|
Gene:
hspa4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783614
[+]
taattaatccccccccccccccccggagatctctcgcggctccattcatctcccccctgcggagaatagcacagtgcggctgcccgccggatctcagtgc
ttcccgctgcccctccgccgtccaccctgcttgtcgctgtgctgtagacatgtcggtggtgggctttgacttgggattcctgagctgctatgtcgccgtc
gctcgggccgggggtatcgaaacggtggccaatgaatacagcgaccgcagcaccccagcatgtatttcatttggtccgaagaatcgatctattggagcag
cagccaaaagtcaagtaatatcaaacgcaaagaacacattacaaggcttcaaaaggttccatgggcgggcatacacagatccatttgtgcaagcagaaaa
gcctggtcttgccttcgaactcgtagagttgccgacaggctctgcaggaatcaaggttgtatacttggaagagaagagatcttttaccattgaacaggtg
actgcaatgcagctaaccaagctgaaagaaactgcagagagtgcgctgaaaaaacctgtagtggactgtgttgtggctgttccctctttctttacggatg
canaaacgcggtctgtagttgatgcccacagattgcagggctgaactgtttgcgccttattaatgaaacactgcaggtgcttttgggcctatgtatttat
aagcaagacttggcctggctcacgaagagaaccccagaattgttggtcttttgcggaaatggggacattcccgcctatcaagtggtctgtctggtgcatt
taaccaggggcaaacctcaagttccttgcccccttccatttgacccctacatttggggtgggcaggaaaatttttgatggaacttttt
3' EST: CF783613
[+]tttttttttttttttggcttgttccaaaacacttcgtagtggtggttcaactcgagataaaaggctgtcacacatttcttcaaaatgccctcgattcata
gatccagtcacatcaatgtcattcatgaaacattcaatattaagtggcagttccgaagcatttgcactcatcaatttcttaagcttctcacactcctgag
caagacgtagcaaaggcctaatcttggacttgatatccagcttgtattttttgccaaattcttcaacgaaatagtttaccaaaacgtcatcaaacttcct
gccacccaatgtagggtcaaatgatgtggcaaggaccttgagtttgcccttgttaaatgcacagacagacacttgataggcggaatgtcccatatccgca
aagaccacaattctgggtttctcttctggagcaggcaagtcttgcttataaataccataggccaaagcaactgcagttgtttcattaataaggcgcaaac
agttcagccctgcaatctgtgtggcatcaactacagaccgccgttctgcatccgtaaagaaagagggaacagccacaacacagtccactacaggtttttt
cagcgcactctctgcagtttctttcagcttggttagctgcattgcagtcacctgttcaatggtaaaagatctcttctcttccaagtatacaaccttgatt
cctgcaaagcctgtcggcaactctacgagtttcgaaagcaagaccagggctttttctgctttgcacaaagggatctgtggtaatgccgccccatgggaaa
cccttttgaagcccttgttaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: