XB-CLONE-4353433
Clone Name: IMAGE:7026165
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Gene:
ctsv
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783616 [+]
taattaatccccccccccccccggcaatttccagcaaaacagcctgagaggatacatcatggcactctatctaggtattgctgctatctgcttaaccaca
gtgtttgctgctccaacaacagatccagctctggataatcattggaatctctggaagaactggcataaaaagtcctatgcaccaaaagaagaaggatgga
ggcgagtgttatgggagaaaaacttaagaatgattgaattccacaaccttgaacactctctaggaaaacattctcatagcctgggaatgaatcagtttgg
tgacatgaccaatgaagaatttagacagctgatgaatggctacaaaaaccaaaagaagataagaggttcaactttccttgctcccaataactttgaatca
cccaagtctgtggactggcgcaaaaaaggctatgtaacaccagttaaagaccagggacaatgtgggtcatgctgggcattcagtacaactggtgcgcttg
aaggtcagcactacaggaatactggtaaaatgatatctctgagtgagcaaaatcttgttgactgctccagagcccaaggaaaccagggatgcaatggtgg
cttaatggatcaagcctttcagtatgttaagggataatggaggcatagattctgaagactcctacccatacacggcctaaagatgaccaggaatgtcact
atggatccgaactacaaattcagccaaatgacccctgggtttttggttgacctttacattctgggaaactgaaaaaagaacttcaatgaaatgccggtaa
cctttcatatggggaacccattctca
3' EST: CF783615 [+]ttttttttttttttttttttttttttttttttttttttttttttaaaagttgaattaacaagtttttattttacattataaaagcttaaatcacataaaa
cacccattaaaatcaacatgtaaacaatactaaaacaacccagcttcaatgtgggatgttaacgtccaacacaaatttaaaaaacagccttgagcagcaa
ttccatcaggtaaaaacagtccagtacttgataaaagtggagtttcacaatatgtgcacataaagttccaaggtcaaaaaggtccatatgcaatggttta
ttttaaaccaaaggataactggcagctgttgctattccacaggggttgtgtctatccttggcaatgtatatgtatccatcgttgccccatttctcgctcc
agctgttctttacaatccagtatttcttgccatcctcatcttcaccctcaaatccatagcccacaacgagtacaccatgatccaggtcttcactgctgca
ttcaggctcataataaatcccaaatttataaaactgaaatgactgatgtcctgcatcaactgcaacagagactggtcccactgaagctactggattcatg
aaatctttcccacttccaaaagtaaagtcaacaaaacccgtggccttggcctgatttgtatttcggaaaaaaaaggacattcccgggtcatcccttaacc
cgggtatggggtaagaaatctttcaaaaaacccatgccctcccattaatcccttaaacaaaactgaaaaagggttttgattcctctttaagcccacccat
ttttgccctcccctggggtttttccttgtgggggtttcttgggagaacatatcttatacaaaagaattttttggcttttctatctcaaaaaagaatataa
tctcactt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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