XB-CLONE-4353427
Clone Name: IMAGE:7026163
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Gene:
lasp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080453 [+]
GGCCTTGCGCGTCGCCGCGGCCCCCGCCCCTCCTGTGTTTGTCACCCGACAGCGCGGCAAAGAGACTGCCAGAGACAGCACACCGGAGAGAGAGAGCCGG
AGAGGGGACCTGTGAGACTGAGTTGAAGGCAGTCCCTCCGTTAGTGGCACAGAGACAGAGGCAGGGACAGGGAGACAGCTGAAGTACCTGGCACCCAGGT
GCTGAGAGACCGGCAACATGAACCCAAGCTGCGCCAGGTGCGGCAAGATCGTCTATCCTACTGAGAAAGTCAACTGCCTGGATAAGTTCTGGCACAAAGC
ATGCTTCCACTGCGAGATCTGCAAGATGACTCTGAACATGAAGAACTACAAGGGATATGACAAGAAACCATACTGCAATGCGCATTACCCCAAGCAGTCT
TTCACCACGGTGGCCGATACCCCAGAAAACCTTCGCTTGAAACAGCAAAGTGAGCTACAGAGCCAGGTTCGTTACAAGGAAGACTTTGAAAAGAATAAGG
GCAAAGGATTCAGTGTAGTGGCCGACACCCCGGAACTACAGAGGATCAAGAAAACCCAGGATCAGATCAGTAATATTAAATATCATGAAGAGTTTGACAA
GAGCCGGATGGGGAACCCATTGGTGGACGGTGATGATTTTGACCGTAGAGGATCAGACGATGGCGCTAATTACCGCAGATCCTCACAGAGTACTCCCCAG
CAGCACCACCCCCCCCCAGCCAGTAGCTCAGCCTATCCGCAGCATCAGCCGCAGCCAAAGTATGGCTACCAGGAACCCGCCGCTCCCGTGTCGTCCCAAC
GCAGCGCCCCAGCAGGAGGGAAGCGATACAGAGCTGTGTACGACTACAACGCAGCCGACGAGGACGAGGTGTCCTTCCAAGATGGCGACACCATCATAAA
CGTTCAGCAGATCGACGACGGCTGGATGTACGGAACCGTGGAACGCACGGGGGACACAGGCATGCTTCCCGCCAACTATGTGGAAGCCATGTGACCCGGA
GGGCCCGCCTCATCTCCTTCTCTCCCAGAATCATGAAGCATTCCAGTGCAGCCCTCTCCTGCGTATTTTGGGAAGGACGGGCTCCGTTCTCTGTATCTCG
GCAATCTTCTCATCCTCTCTGCGGCCCCATTGGCTGTAGGGCGGCTAATTACCTCTCCGCTTCAATCTATTTTCCGAAGGGATCGAGTTGGGAAATATTT
CCGTTCCCACAGCCGTTTCCCCCCACTCCTGTAATGTCCAATTGACATTTCTCCCTATGCGCTTTGCACTGAATATATATATATAATATATCCCTAATAT
ATTATATATATAAAGTAGATCATAAAGTGGAGGCAAAGGGAACCTGATAATCTTTTCAGACGAAAGCATCAGTATCTTTCCATCCTTCCAGTTGGAAGGC
ATCGCTGCCCGCTCTGGTCATCCAAAACGACTTTCTGCATATTTTTATTCTCGTTTTTCCTCCCCCCCCCCCCCCCCTCAATCTGGGTAAATGACACTTT
GTTCTATTTACCACGAGAAGTTTAGGATTTTTACTATTCACAAAAGCTGTGATGACCTTGCTGTGAGCGAGCGTGGATCTTGTTAGCGTGACGGAACTCG
GTCCTGCCCCGAGGGCCACATTCCCCAGCACCGTCAAACATGAGCGTAATAGAAGCAGGATGGTATCGGCCGCCATCCATCAGTAGAAATCAGTGGCTAA
AGGAACCACAAGCGATTAACGGTTTTAATCAATTTGATTATGATTTGAATCTGCCAACGAGCAGTTTATATACCGGTGGGGGCCGCGTCGCCCCCTGTCC
CGCCTCATAGATAGAGTACGCCCCTGCCACGGCACGTGCCATTCAATGACATTAACATCGAAACATAAAGACCGCAAAAAAAAAAAAAAAA
5' EST: CF783620 [+]taattaatcccccccccccccggccttgcgcgtcgccgcggcccccgcccctcctgtgtttgtcacccgacagcgcggcaaagagactgccagagacagc
acaccggagagagagagccggagaggggacctgtgagactgagttgaaggcagtccctccgttagtggcacagagacagaggcagggacagggagacagc
tgaagtacctggcacccatgtgctgagagaccggcaacatgaacccaagctgcgccaggtgcggcaagatcgtctatcctactgagaaagtcaactgcct
ggataagttctggcacaaagcatgcttccactgccagatctgcaagatgactctgaacatgaataactacaagggatatgacaagaaaccatactgcaat
gcgcattacctcaagcagtctttcaccacggtggccgataccccagaaaaccttctcttgaaacagcaaagtgagctacagagccaggtttcgttaccag
gaatactttgaaaaccaataaggggccaaggattcagttgtaggggccgaaaccccgggaactacttaggatccagaaaaaccctggatccagatcccgt
cattttacatttttccggaatacgtttgaatttgaaccccggatgtgcgcaaccccccttggggggaacgggggatgtatttcggacccataggtaggct
ttacgaacaatggccgcctaattttacccccttatatccctcttgaaaaggccttcccccaggttgaccacccaccttttccccccaaatccggatagtc
ttcatgcccctttctcgggtgcttttcccgccccgccagcccctattccccttgcccctcccctcgggaaccccc
3' EST: CF783619 [+]tttttttttttttttgcggtctttatgtttcgatgttaatgtcattgaatggcacgtgccgtggcaggggcgtactctatctatgaggcgggacaggggg
cgacgcggcccccaccggtatataaactgctcgttggcagattcaaatcataatcaaattgattaaaaccgttaatcgcttgtggttcctttagccactg
atttctactgatggatggcggccgataccatcctgcttctattacgctcatgtttgacggtgctggggaatgtggccctcggggcaggaccgagttccgt
cacgctaacaagatccacgctcgctcacagcaaggtcatcacagcttttgtgaatagtaaaaatcctaaacttctcgtggtaaatagaacaaagtgtcat
ttacccagattgagggggggggnnggggggggaggaaaaacnagaataaaaatatgcanaaagtcgttttggatgaccagagcgggcagcgatgccttcc
aactggaaggatggaaagatactgatgctttcgtctgaaaagattatcaggttccctttgcctccactttatgatctactttatatatataatatattaa
gggaacatcataaatatataatccattgcccacgcgcataagggagaaaatgtccattggacatttaagggattgggggggaatcgggctgtggggaacg
gaaaatattttccccaactcctattcctttcggaaaaataaatttgaaccgcgaaaagggtaatttggccggccccttaaagccaattgtggggccgcca
aaatagcgaagagaaaaatttttcattaatatcacagaaacggaacccccgccccctttcctaaaaatatccaggggaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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