XB-CLONE-4353424
Clone Name: IMAGE:7026162
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Gene:
syt4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077008 [+]
CGAGCCATTAGCAGACAGGACTCCCATTATCGGCAAAGCAAGATCCAGCGGCTGATTATTTCCCTGACTGGCTTTATCTCGAGCCACTGAGCATCCTCCG
GCAGAGAGAGCAGGAGCGGCTGAGATCTCTTTCGGCACCTCGGACGCCACAAGCAGGAAAAATGGCTCCAATCACGAGCGGCAGAGATGCTTTTGATGAA
ATTCCATCAGTGGTTGGACTGGTCAGCGGGTTTGGACTTGTTTTCACGGTGTCTTTGTTCTCGTGGATTTGTTGCCAGCGTAAATCAGCCAAATCCAACA
CGACGCCCCCGTACAAGTTTGTCCATGTGTTAAAAGGCGTTGACATATACCCCGAGAGCTTAAACAGCAAAAAGAAATTCGGAGCAGATGAAAAAACAGA
ACTGGCTCCTAAATCTCTCATCTCAAAGACTTCTCTTCACCTTGATCTCGAAAAAAGGGACTTAAATGGCAATTTTCCGAAAGCCAATAAAGTCAGAAGC
TCTCCAGACCTTGAGGATTTCTCGCAGCCTCAATTACCAAAAAAAAAAAAAAAA
5' EST: CF783622 [+]taattaatccccccccccccccgagccattagcagacaggactcccattatcggcaaagcaagatccagcggctgattatttccctgactggctttatct
cgagccactgagcatcctccggcagagagagcaggagcggctgagatctctttcggcacctcggacgccacaagcaggaaaaatggctccaatcacgagc
ggcagagatgcttttgatgaaattccatcagtggttggactggtcagcgggtttggacttgttttcacggtgtctttgttctcgtggatttgttgccagc
gtaaatcagccaaatccaacacgacgcccccgtacaagtttgtccatgtgttaaaaggcgttgacatataccccgagagcttaaacagcaaaaagaaatt
cggagcagatgaaaaaacagaactggctcctaaatctctcatctcaaagacttctcttcaccttgatctcgaaaaaagggacttaaatggcaattttccg
aaagccaataaagtcagaagctctccagaccttgaggatttctcgcagcctcaattaccaaaaaaaaaaaaaaaag
3' EST: CF783621 [+]tttttttttttttttggtaattgaggctgcgagaaatcctcaaggtctggagagcttctgactttattggctttcggaaaattgccatttaagtcccttt
tttcgagatcaaggtgaagagaagtctttgagatgagagatttaggagccagttctgttttttcatctgctccgaatttctttttgctgtttaagctctc
ggggtatatgtcaacgccttttaacacatggacaaacttgtacgggggcgtcgtgttggatttggctgatttacgctggcaacaaatccacgagaacaaa
gacaccgtgaaaacaagtccaaacccgctgaccagtccaaccactgatggaatttcatcaaaagcatctctgccgctcgtgattggagccatttttcctg
cttgtggcgtccgaggtgccgaaagagatctcagccgctcctgctctctctgccggaggatgctcagtggctcgagataaagccagtcagggaaataatc
agccgctggatcttgctttgccgataatgggagtcctgtctgctaatggccggggggngggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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