XB-CLONE-4353421
Clone Name: IMAGE:7026161
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Gene:
rps7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783624 [+]
taattaatcctccccccccccgctcttcctaggccgtcactgagacaggacgaaaggccatgttcagcacaagcgctaagatagtaaagccgaatggcga
aaagccagacgagtttgagtcgggtatttcccaggctctcttggagctggaaatgaactctgaccttaaagctcagttaagggaacttaacatcacagct
gcaaaggaaatagaagttggaggtggtagaaaagcaattatcatctttgttccagttcctcagctgaaatctttccagaagatccaagtaaggctagtgc
gcgagttggagaagaaatttagtggcaaacatgttgtcttcattgctcagagaaggattctccccaagccaacaagaaaaagccgcacgaaaaacaagca
gaagcgtcccagaagtcgcactctgactgcagtacatgatgccattctagaagacttggtttacccaagtgaaattgtgggcaagagaatccgtgtcaaa
ctggacggcagtcggcttatcaaggtccatttggacaaggcacaacagaataatgttgaacacaaggtagtggaaacatttttctggtgtgtaccacaaa
ctcacaggcaaagatgtggtcctttgaattcccctagttcccagctgtaaatgcgccaggattgcattattaaaaaggaccttcttgtttgtggccataa
ggaccccaagccgaggaaaaaatttgtactcgcaaggggttccccccttaattcttagtaccgggccggctcaaccccggggggaaaactttgggccctg
tgggcccccacagttgggtttaactctgccccccttttttatacgggcggtttccccaattaaaaaggccgatta
3' EST: CF783623 [+]tttttttttttttttctttttttttttttttttttttttaacaagaagtactttttattatgcaatcctgcacattacagctggaactctgggaattcaa
agaccacatctttgcctgtgagtttcttgtacacaccagaaaatgtttccactaccttgtgttcaacattattctgttgtgccttgtccaaatggacctt
gataagccgactgccgtccagtttgacacggattctcttgcccacaatttcacttgggtaaaccaagtcttctagaatggcatcatgtactgcagtcaga
gtgcgacttctgggacgcttctgcttgtttttcgtgcggctttttcttgttggcttggggagaatccttctctgagcaatgaagacaacatgtttgccac
taaatttcttctccaactcgcgcactagccttacttggatcttctggaaagatttcagctgaggaactggaacaaagatgataattgcttttctaccacc
tccaacttctatttcctttgcagctgtgatgttaagttcccttaactgagctttaaggtcagagttcatttccagctccaagagagcctgggaaataccc
gactcaaactcgtctggcttttcgccattcggctttactatcttaacgcttgtgcctgaacatggcctttcgtcctgtcccagtgacggcctaagaagag
cgggggggggggaggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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