XB-CLONE-4353418
Clone Name: IMAGE:7026160
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Gene:
atp5f1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783626 [+]
taattaatcccccccccccccgcagagctacagcctctgagtgctccttctgcactgctgctgctctccgctgctgacgccgccatgttaggggctgtag
gacgttgttctgcgggggcactgcgggctctaaagccagcatctaacccagttcagctcgggcagaaccttctcagatgttcccctgcagcgctgcactc
ccggagagattatgcagctcagacttccgcagcagccaagcctggaacagcagtcggccgcattgtggcagtcattggtgctgttgttgatgtccagttt
gatgaaggcctcccacctatcctgaatgccctggaggtccagggcagagacaccaggcttgtgctggaagttgcacagcatctgggtgaaaacactgtcc
gaacaattgctatggatggcacagaaggtttggtcagagggcagaaagtccttgatgctggcacaccaatcagaattcctgttggtcctgagacccttgg
aagaatcatgaatgtaattggtgaacccattgatgaaagagggcctattactaccaaacagtttgcagccatccatgcagaagccccagagtttgtggag
atgagcgttgagcangaaatcttggttactggcattaaagttgtagatctgctggcaccctatgccaaaagaggaaaaattggtctgtttggtggtgcag
gagtangtaaaaatgtgctaattatggagctgatcaacaatgtagccaaagctcatggtgggttactcctgttgtttgctggaattggaaaaccgtaccc
ctgaaaggaaatgaatttgtaaccatgaaattgattggaatccgggagtaaaccaacttggaaaggataacaaccct
3' EST: CF783625 [+]tgatacgagatgttgagtccagaggatccacagcaggataaatacccaactcagcaatagcacgagacaatactgtagtagcgtccaaatgggcaaaggt
ggtagcaggagctgggtcagtcaagtcatcagcaggtacataaatggcctgcacagatgtaattgaccctttctttgtagttgtaattctctcttgcatg
gtacccatgtcagtggccagagttggctggtaaccgacagcagaggggatacgtcccagcaaggcagacacctctgaaccagcctgggtaaacctgaaaa
tgttgtcaataaaaagcaacacatcttgtccctcttgatctctgaaatattcagcaacagtcagaccagtcaaagcaacacgagctctggcacctggggg
ctcgttcatctgcccatataccagtgcgacctttgaggttgtatccttcaagttgattactccagattcaatcatttcatgatacaaatcatttccttca
cgtgtacgctctccaactccagcaaacacagagtaaccaccatgagctttggctacattgttgatcagctccataattagcacagttttacctactcctg
caccaccaaacagaccaatttttcctcctttggcatagggtgccagcagatctacaactttaatgccagtaaccaagatttcctgctcaacgctcatctc
cacaaactctgggggcttctgcatggaagggctgcaaactgtttgggtagtaataggcccctcttttcatcaaggggggttccaccaaatttccattcct
ggaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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