XB-CLONE-4353410
Clone Name: IMAGE:7026157
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Gene:
yipf5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783631 [+]
taattaatccccccccccccccgtgcgcggcctgatagagctgccaacatgtcgaactttgataatttcaacacagatttctatcagaccagctatagta
tagatgatcagtctcagggctacaactacaatgcaggcggagcacaacacagcaagcaatatgcttatgatccctacagtcagcaaggaggttttattcc
accagagatgatgaatcaacagcagccatacacgggacagatataccaacctacacaaacatacactcctgcagccactgactctgtgtatgggagtaca
tttgatgatgagccacctcttctagaagaactgggaatcaactttgaccacatttggcagaaaactctgactgtgctacaccctttaaaagtagcagatg
gcaacattatgaatgagacagatttggcgggtccaatggtcttctgtctagcatttggagccacgttattgttggctggaaagatacagtttggctatgt
ctatgggatcagtgcaataggatgtctgggaatgtattgtcttctgaatttaatgagcatgacgggcgtttcgtttggctgtgtatccagtgtacttggt
tactgtcttctttccatgattatactctccagttttgctgtaatctttcctttacaaggaattttgggcattgtttcttgctgccttgaataattggttg
ggggcattttctccgccttcaaagaacttccatttctgccctgggccagggaatgagaaaacgggttttaagttgccctaacccctggtgcttttacttt
caaggggaaggggttgtgccccctaaacatccgggggttttggac
3' EST: CF783630 [+]tttttttttttttttgtgactcaattttttttttttattactgtaaataatttttcatactgtgctattatcagtgaaacatcaccatagcatgcatgtt
ccttctgtgaacaccaccaaacattacattgttaccttacaaccaggcattttattaacaccgaagacattgggagtcccttcagtattaacagttctgt
ataaaagctgcgaaccagatgttcgttatttccattgttgtcccagagacattagaatgtgagatagcaaagctctctggtcctttctgggagacaggat
tggtctttattagctgttggcccactaaagtccaaattaaaataaaatatgctcaaaacacagagataagagcaaacactccataaagtaaagcacaagg
gtaggcaactagaacctgttgtccatccatggccagagcagaaatgaagatctttgaggcggagaaactgcaccaaccaattatcaaggcagcaagaaca
atgcccaaaattccttgtaaagagaagattacagcaaaactggagagtataatcatgggaagaagacagtaaccaagtacactggatacacagccaaacg
aaacgcccgtcatgctcattaaattcagaagacaatacattcccagacatcctattgcactgatcccatagacatagccaaactgtatctttccagccaa
caataacgtggcttcaaatgctagacagaagaccattggacccgccaaatctgtctcattcataatgttgccatctgctacttttaaaggttgtagcaca
gatcagagtttttctggccaaatgttggtcaaagttgaattccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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