XB-CLONE-4353398
Clone Name: IMAGE:7026151
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Gene:
snrpe
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077697 [+]
CGACTTCCGGTTCGGCTCATTCCTTTGGATTCACGAGGCGGCAGAGATGGCGTACCGCGGGCAAGGCCAGAAAGTGCAGAAAGTGATGGTGCAGCCCATC
AATTTGATTTTCAGATATCTTCAAAATAGATCACGAATTCAGGTGTGGTTGTATGAGCAAGTGAATATGCGTATCGAAGGCTGCATCATTGGTTTTGATG
AATACATGAATCTAGTGCTGGATGATGCAGAAGAAATCCACCTCAAAACAAAATCTCGCAAACAGCTTGGACGTATCATGTTGAAAGGAGATAACATCAC
TTTACTACAGAGTGTCATGAATTAATGCTTTGGATTAATCTGTTCTCCACCGCTGTTCCCATACATCTTCATCAGTGAAGAAAGGAAGAATTAACCAGTG
TACTTATTCCCCCTTTTGTGGACTTTTTCAACTGTGTTGTAGATTTCTAGCTGTTATGATGCCCTCAAGGATTACAATGTAACTTTTTTTTTTATATATT
TTTAAAATCAAACTATTTCAGATGTTGAAGGCAGTTGCATAAAAACTAATGTATCTGCAAGGATTTGAGTTGTGTACAATTGTACAACTAGGTTAAAGCA
TTTTTTTTTCCCAGAGAAACTAAACGTGATAGGGCTTAGAGCCCATGAAGTGGGTTTCCCCAGAGTATGTTTTTGATACTGAGTTCCAAATAAACCAAAT
GTTTTTGGAATTGTTTGTGTAAAAAAAAAAAAAAAA
5' EST: CF783639 [+]taattaatccccccccccccccgacttccggttcggctcattcctttggattcacgaggcggcagagatggcgtaccgcgggcaaggccagaaagtgcag
aaagtgatggtgcagcccatcaatttgattttcagatatcttcaaaatagatcacgaattcaggtgtggttgtatgagcaagtgaatatgcgtatcgaag
gctgcatcattggttttgatgaatacatgaatctagtgctggatgatgcagaagaaatccacctcaaaacaaaatctcgcaaacagcttggacgtatcat
gttgaaaggagataacatcactttactacagagtgtcatgaattaatgctttggattaatctgttctccaccgctgttcccatacatcttcatcagtgaa
gaaaggaagaattaaccagtgtacttattcccccttttgtggactttttcaactgtgttgtagatttctagctgttatgatgccctcaaggattacaatg
taacttttttttttatatatttttaaaaccaaactatttcagatgttgaaggcagttgcataaaaactaatgtatctgccaagatttgagttgtgtacaa
ttgtacaactagggttaacgcattttttttttccccgaaataaccaaacggtgtacccggcttaacagcccaatgtaaggggggttttccccagaaagaa
cgtttttttgaaaacctgagttccctaattaagcccaaaagggctatttgggaactcgtctccgggggcaaaacaaacaaacaattaaaccgattccccc
ctttgtttccctataaccccgggctgcgcttcacctttcggggtggagaacttttgtgttcccgcaggttccacacctttcgcgtttttatt
3' EST: CF783638 [+]tttttttttttttttacacaaacaattccaaaaacatttggtttatttggaactcagtatcaaaaacatactctggggaaacccacttcatgggctctaa
gccctatcacgtttagtttctctgggaaaaaaaaatgctttaacctagttgtacaattgtacacaactcaaatccttgcagatacattagtttttatgca
actgccttcaacatctgaaatagtttgattttaaaaatatataaaaaaaaaagttacattgtaatccttgagggcatcataacagctagaaatctacaac
acagttgaaaaagtccacaaaagggggaataagtacactggttaattcttcctttcttcactgatgaagatgtatgggaacagcggtggagaacagatta
atccaaagcattaattcatgacactctgtagtaaagtgatgttatctcctttcaacatgatacgtccaagctgtttgcgagattttgttttgaggtggat
ttcttctgcatcatccagcactagattcatgtattcatcaaaaccaatgatgcagccttcgatacgcatattcacttgctcatacaaccacacctgaatt
cgtgatctattttgaagatatctgaaaatcaaattgatgggctgcaccatcactttctgcactttctggccttgcccgcggtacgccatctctgccgcct
cgtgaatccaaaggaatgagccgaaccggaagtcgggggggggggggggggattaatttaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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