XB-CLONE-4353391
Clone Name: IMAGE:7026148
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Gene:
apoe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783643 [+]
taattaatcccccccccccccgattgctattacaggcaagaagaacaatgaagctgatgatcctcttgctcacgcttgggctcctggcaggggcccaggc
cagatctctcttccgagatgcaccaaagacaaaatgggagactgccctggatagtttctggctaacagtcaacaaaatggaggaggcagctgatgaggcg
agagccaaaatgaaggattcaccaatcagtaaagagctggatggccttatcaaagacacaatggaagaactgagcatttatgcagaagacatgaaaagta
aggtgagccccatggcccaggatgcacagcagcgctttactgaggaggtatctgccctggctgataagctcaagtctgacatggaggatacaaagaacaa
agccattcagtactcaggagacctgcgcatgatgtttgatcagaacattgaggatgtccaaggcaaggtgaacatgtacatgaagaaactgaagaaacgg
ctgagtaaagacagtgaggatttaaaaaagaagctcagccaatatggagaagatttccgtaagagtactgaagacaaagtggaaaatctccgtaaagccg
tggagccttatttgtccaacgtgaaagataaagggcagcaaaggctgatgaacctgaaggatgccataaatgagccaagggaaaacaacttcaaaaaccg
ggtttgtcctctgtttccaaaaaaacccccaaagctaaaaatgaaagaagaaaagcccttctggaagttgaaaaaaagcagtgggggggaaaaaaactta
ggggggaaaaaaggtttcccccgttaaaatggggttctaaaaccccccccaatgggttaaaaaaaaagcccccttggcccccaanaatccaaaggctttt
tctaaaaggcacc
3' EST: CF783642 [+]taatgtcctgctccacctcagggcgcaatcttatggtcaaaagtagcagctgcaagtgagaagcaaggaaaaacactggttctttcatttatttgttggc
cttggtctgcaggctcttagccaaggcttgaagctgattgcgcaggctctctacattggggttgaaccatttacggaactgttcccctagtttatccaca
ctgcttttcacatcagaggctgtcttcttcattttagcttgggcgtctttggcaacagagacaaaccggtctctgagttgtttcccttgctcatctatgg
catccttcaggttcatcagcctctgctgccctttatctttcacgttggacaaataaggctccacggctttacggagattttccactttgtcttcagtact
cttacggaaatcttctccatattggctgagcttcttttttaaatcctcactgtctttactcagccgtttcttcagtttcttcatgtacatgttcaccttg
ccttggacatcctcaatgttctgatcaaacatcatgcgcaggtctcctgagtactgaatggctttgttctttgtatcctccatgtcagacttgagcttat
cagccagggcagatacctcctcagtaaagcgctgctgtgcatcctgggccatggggctcaccttacttttcatgtcttctgcataaatgctcagttcttc
cattgtgtccttgataaggccatccagctctttactgaatggtgaatccttcattttggctctcgcctcatcaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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