XB-CLONE-4353386
Clone Name: IMAGE:7026146
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Gene:
pgrmc2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077054 [+]
CACGCTGCTTGCGCGGCCGCTGCCCTCTAGTCCCTTTGCTCCGCCCCCTGCACACGGGGATCGTGGGATTGGCGCTGAGTACTTTCCCAGCTGGCCTTGC
CGTGTGCTGTGGGTACAGCGCCAGCTCTTCCTAGTGTCCTGCAGAAGCCCTCCCTGGTGCGGGAGTCAGGTGGCCAGCGGTGACTCGGGTGCTGCCCGGC
AGGTAATGTCGGAGAGCTGGGAGGCGGAGGAGCAGCTGGCTGGTACCTCGCCACCGGGCCGCGAAGGAGAGCCGTCCGGACTAGGATGGCTATTTGCTGG
GGAGCTGCTGCTAAACGCGGCTCTTGTGCTTGTGGTGGTATACGGGGCCTTTCGAATCTACCTGCGCTGGAAAGGGTCGGGGTCGGGCGGAGTCGCTTCT
TTGCCCCGTATGAAACGCAGAGACTTCACCCTGCAACAACTGCAAGAGTATGACGGGACGCGCAACCCCAGGATCCTTCTGGCTGTCAATGGGAAAGTGT
TTGATGTCACCCAAGGAAGCAAGTTCTATGGGCCGGATGGCCCCTATGGGTTATTTGCTGGCAGAGATGCTTCCAGGGGACTAGCAACATTTTGCCTCGA
CAAAGAAGCACTCAGAGATGAATATGATGACCTATCTGACCTAAACGCTGTACAGATGGAAAGTGTTCGGGAATGGGAAATGCAGTTTAAAGAAAAGTAT
GAATACGTTGGTCGACTGCTAAAACCAGGTGAAGAGCCATCTGAATATACAGATGAAGAAGATGTCAGAGACCATACAAAGCAGGACTGAACTTTGTTAC
CAGCCAAAGTCAGGGGCCGTTCAGGAATTGCGTTCACTTTCCCTACAACATATAATGAGGAGATTTTGTGCTCCATGATATTCGCCACAGTTTGTACAGG
GAAATGAGAATTAAACGTGATACTGCAAAAGAAGATTTTAATACTAGGCATTATGATCTTCACTGCCAAGCATTTTTCTCCCCCCTACAAAAGTTGATCT
TTCTGTGACCGAACCTTATTAATGAAGCAGGATTCAGGAAATCAGTTTTGTGAAATGTGCATTGTTACATATTTTATTAGTACACTCTAACTGGCATTTG
TGGAGCAGCTCCACTTTGGCTTTATGAGGAACCAGCCATGATTCTGCCAAATGAGCAGTTCACAGATCAGAAAGGGTATAGAAAAGTATACATTTTTTAT
GTGGTATTTGGTTCAGACTTTTGAAAACCTGTTTTTTTTTTGTTTTCCATAAATACATTTATCAGATTAAGTCTGCACTACTTAGAAAAAAAAAAAAAAA
A
5' EST: CF783646 [+]taattaatccccccccccccccacgctgcttgcgcggccgctgccctctagtccctttgctccgccccctgcacacggggatcgtgggattggcgctgag
tactttcccagctggccttgccgtgtgctgtgggtacagcgccagctcttcctagtgtcctgcagaagccctccctggtgcgggagtcaggtggccagcg
gtgactcgggtgctgcccggcaggtaatgtcggagagctgggaggcggaggagcagctggctggtacctcgccaccgggccgcgaaggagagccgtccgg
actaggatggctatttgctggggagctgctgctaaacgcggctcttgtgcttgtggtggtatacggggcctttcgaatctacctgcgctggaaagggtcg
gggtcgggcggagtcgcttctttgccccgtatgaaacgcagagacttcaccctgcaacaactgcaagagtatgacgggacgcgcaaccccaggatccttc
tggctgtcaatgggaaagtgtttgatgctccccaaggaagcaagttctatgggccggatggcacctatgggttatttgctggcagagatgctttcccggg
gacttagcacattttttcctcgaccaaagaagcactcagaagatgaatatggacgaacctatccggaacttaaaccgctggtacagaatgggaaaagggt
ttccggggaatcggccaaaatgcctcctctctaattacaaacttattcatattaattctttggtcgaaacctggctaaaatacctggggtgtaaagagag
ccattcttcgtacattttattctccacccaaatcgacaaacaattgtttccaaaaacgcacccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: