XB-CLONE-4353383
Clone Name: IMAGE:7026145
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Gene:
snrpe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783647 [+]
taattaatccccccccccccccccgtcattcctttggattcacgaggcggcagagatggcgtaccgcgggcaaggccagaaagtgcagaaagtgatggtg
cagcccatcaatttgattttcagatatcttcaaaatagatcacgaattcaggtgtggttgtatgagcaagtgaatatgcgtatcgaaggctgcatcattg
gttttgatgaatacatgaatctagtgctggatgatgcacaagaaatccacctcaaaacaaaatctcgcaaacagcttggacgtatcatgttgaaaggaga
taacatcactttactacagagtgtcatgaaattatgctttggattaatctgttctccaccgctgttcccatacatcttcatcagtgaagaaaggaagaat
taacccggtgtacttattccccccttttgtggactttttcaactgtgttggtagatttctaggctgtttatgatgcccctcaaaggatttacaattgtta
accttttctttttttaattataaatttttaaaaattccaaacctattttttcaaaattgttggaaaagggcaaatttgccaattaacaaaaccctaaatt
tggtattccttggcgaagaggggaaattttagaaagatttgggtggctaacaaaaaattttggtgttgccaaaaaaccttgtaggggggtttttataaaa
gagcctacacagtgtctctatttgttttttttcccccctcagaaaaagtataaaaacctttaataaaatcgcgtttgggaaaagattggggccccttttt
taaaaaaaacccctccaatttagaaaaaaggtgtggggggtttttttcctccccccccaaaaaaaataatagggtttttttttgggggagaacacccggg
gaaagtttttcccacaatatatttaaatcgctcaatcaagtggggttttctttttggggggtcgcaaagagtagcctgaatattactatggaggggggta
ttctccccctcttganatgttctcttaaagaaaagagaggaggaatctcgatccacaacactcgcttcgattgtgggagaacactacttgagtcctctag
gtggagactctccaaactcttgtggtatttattatatgcgcacgattcctttaaataaaaaagagggttctacccaacaaa
3' EST: CF783648 [+]tttttttttttttttccaaaaaatttggtttatttggaactcagtatcaaaaacatactctggggaaacccacttcatgggctctaagccctatcacgtt
tagtttctctgggaaaaaaaaatgctttaacctagttgtacaattgtacacaactcaaatccttgcagatacattagtttttatgcaactgccttcaaca
tctgaaatagtttgattttaaaaatatataaaaaaaaaagttacattgtaaatccttgaggggcatcattaacagcctagaaatcttacacaacagttta
aaaaaagccaccaaaaggggggaataaagtacaccgggtttaaattcttcccttttctttccctggataaaaaaatgtttatgggggaaaaaaacccggg
gggggggaaaaacccattttttttttccccccaaaggagctttaaaatttttttttattaaaaaccccctctcctgtttaataaaaaaaaaagggggggg
gggttttantctcccccctcttttttccaaaaaaggtggtataataagcccttccacaacaaaaatattttctttttgtttctatacgagaaagaaaaaa
aaaatgttcgtgtggttttattttagagaggaggggagagagaaaaaaaaaattattttctctttggggggcgggggggagctaactctgttcacccgcc
nccggggatcgaataagagaaaagaacactaccgctgggtgtggtgttcattttttctcccccaaaaataaacacaacacaaacaataagagtgtggtgg
gtggtccctcctctctcccttctttattaaataaaccgacccaaatatgttattttcctcgtggtggggggtggccggcnagaaaaaaagacacacccac
cacccctccttctttaaaatatatatcgtgggggcgccgtatgtctatttttntgtgttgatagaaagacaatatattcttcgcataaaaaactaaacac
attccttttgttatgggggggggggggcggtgacacacaactgtcctaccttttatttgtggggtactaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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