XB-CLONE-4353380
Clone Name: IMAGE:7026144
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783650
[+]
taattattcccccccccccccatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctataggt
ccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggaggagg
ctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaatgaa
aagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatccgca
cttggcacgagaagcaaaccggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaagatccatgg
tgcaactgttgataacgctacagtgaccctgcaaatcgacaacgccagactggctgctgacgacttcagacttaagtttgagaatgagctggctcttcgc
cagagcgtggaggctgatatccctggcctgcgtaaagtcctggatgaactgaccctggctaaaggtgacctggagatgcagatcgagagccttgactgga
aaagcttggccttacctgaaaaaaggaacccatgaaggagcgaaaattaatgcattggccaaagagacccagaagccgccggggcaaaaaatgaagttgg
ttgtaaaaatgggaacccccgtttccccccagggcgttgggaatttttgtaaccaaacaattc
3' EST: CF783649
[+]tttttttttttttttctttttttttttttttttttagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcagg
tcttctctcttgtttcaggggaaatttctgttcatttgttcctttatctttcccgactgcccttgcatttatgacactgttgaagaaacgaccttcccat
ctaccacctcttcgacaatggtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgt
ggtcatctgtcccagttccccatccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttc
tgatgttccatgtcggacctgatctgcagaagctgttcctctaggctgctgatagtgttctgaatctgtagaagttgcgctgaatagaagccttgtaatt
cattgaggttgccttcaactgattgtttcattgccagctgggattgcagctcaatttccaagctctgaagtgaccgtttcagctcagtgatttcgctctt
gctggcctgcacttgttcaaccccaactgaaatctctttcttcagctcaccgctcttctgggtgaaccacaactctgcatccctgcggttcttctcagcc
agtatttcaaagtcagctctcatgttattcagaaagttggtcagatccccgcctggagccgcgtcattctcaacattcacttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: