XB-CLONE-4353367
Clone Name: IMAGE:7026139
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Gene:
stk25
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783657
[+]
taattaatcccccccccccccggccctgcctcctcctcacccacatgctccagggagtctcctgtgtcagtaagcagtgctaacgagggacaagacatgg
tgtcagcagctgcagtgctagcagggatctacaagcaaagtaccacagaactagctctaggatggatcctgagcgtcttttcaccaagctggagcgcatt
gggaagggctcctttggagaggtgtataagggcatagagaatcgcagtaaagaagttgtagccatcaaaattattgacctggaagaggcagaggatgaaa
ttgaggacatacaacaggaaattacagtgctgagccagtgtgacagtccttatatcactcgctattatggttcctacctgaagggatcaaagctatggat
aataatggagtatttgggaggaggctctgctctggacctgctgaaaccaggccctctggaagaggcgtacattgccactatactacgagaaatcttaaaa
ggacttgattatttgcattcggagagaaagatccatcgagacattaaagctgcaaatgtgttgttatcagagcagggtgatgtgaagttggcagattttg
gtgtagcaggtcagctgactgacacccagataaagaggaacacatttgtggggaaccccattctggatggcgccctgaagtcattaagcagtcagcccta
cgactttcaagggcagaacatctgggtcttttgggggatcaccgcccattggagcttgggccaaaaggagaaccccttccatacctcaggatcctttcac
cccctattggagggggtccttggtttc
3' EST: CF783658
[+]tgttcagacatgcctcaacaaaatctttgaatggtttactgtactggccctggagagacggaggattgttcttaggaatcaggaacaggaccctcatagg
gtgaagatctgagtatggaggctctcctttggccagctcaatggctgtgatccccaaagaccagatgtctgccttgaagtcgtaggctgactgcttaatg
acttcaggcgccatccagaatggggttcccacaaatgtgttcctctttatctgggtgtcagtcagctgacctgctacaccaaaatctgccaacttcacat
caccctgctctgataacaacacatttgcagctttaatgtctcgatggatctttctctccgaatgcaaataatcaagtccttttaagatttctcgtagtat
agtggcaatgtacgcctcttccagagggcctggtttcagcaggtccagagcagagcctcctcccaaatactccattattatccatagctttgatcccttc
aggtaggaaccataatagcgagtgatataaggactgtcacactggctcagcactgtaatttcctgttgtatgtcctcaatttcatcctctgcctcttcca
ggtcaataattttgatggctacaacttctttactgcgattctctatgcccttatacacctctccaaaggagcccttctcaatgcgctccagcttggtgga
aaagaacctcaggaatcatcctaaaagctagttctgggggtacttttgcttggaaaatccctgcctaacactggcagcctgctggacaacaatggtcttg
gtcccctcgcttatgcccatggctttactgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: