XB-CLONE-4353362
Clone Name: IMAGE:7026137
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Gene:
krt7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783660
[+]
taattaatcccccccccccccaacaagtatgaagatgagatcaacaagcgcacttccgctgaaaatgaatttgttgttctgaagaaggatgtggatgctg
cctacatgaacaaagtagagctggaggccaagatggaggctctgacagatgagatcaacttcctgagggccctgtatgaagctgaactccgtgagctgca
ggaacagatctctgacacttcagtcgtcttgtctatggacaacaacagagctctggacatggacagcatcattgccgaagtcaaggctcagtatgaagaa
attgccaacaagagccgtgccaatgcagaggctgtgtaccagaacaagttccaggaactgcaggccaccgctgggcgacatggtgatgacttgcgcacca
ccaagactgagatttctgaactgaaccgtgctatgcaaagactgcaggctgagattgagagcgtaaaggctcagagagctaagcttgaggcacagatcgc
agaggccgaggaacgtggagagctggcattgaaggatgccaggaccaagctggctgagctggaggctgctctgcagaaggccaagcaggacatggctcgc
caactgcgtgaataccaggaactgatgaatgtgaaactggcccttgatattgagatcgccacctacaggaaactgctggaaggagaagagaccagaattt
ctgaaggacctggaccagtcagtgtatctgtggtgaactcaacctccagcagcatgggcggcagcagcagtggatacggtgctggatatggttctgggat
cc
3' EST: CF783661
[+]tttttttttttttttagattgaaggaatttttattggagttgcttgtgtttctcaccagacataaaatccctcccacagaattcagatttccaccacaaa
cacagaccatatctaaaagagtggtaactaaaacagaattgttccatatactgggtgttttacaactgcaaacaaacattaaggaatcactgttctttgt
tttcccatctaagctgccagatttacatcagagacttactagaatttgttttacgttatatgttagcaccgtatatctcttttgttacctatcaagcctc
ttctatgggaaaacaaaacaaacacaaagatatctgcccagaaatactgctaattttttatgtatttgttttaatacaggcatgagatttccattgtgaa
ctgagcaatacaacttgcagcggtcgatactgtaaaaacgtgcccagacttatggcgaataggacacctagattcagcatacgattagagccagtcccac
tggtagaagggggagcagtgaaggagaaagatgcaagtaggcacttgtagacaggaatagggaactgctttcaagatttctgcaaacagccatatcctcc
agataataacaaacaacaaaatgcgggtgctagactacacacttggaaaggtgcccacaagatataggtataaggggtataaccccactaagtaataccc
ttaccaacaacaacacattgtaaacagagcctgttttgaatattttgagggctatgggctaatggctcctaccatcaatacacagggggacacctttttt
tctgctgccggcacatcctaattgtcctaaagcctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: