XB-CLONE-4353356
Clone Name: IMAGE:7026135
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Gene:
tubb4b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783664 [+]
taattaatcccccccccccccccccgagcgctccaaggtaaccggttcagttcggagtttagtacagagcaaccaactacagtcgccatgagggaaatcg
tgcatctgcaggccggccagtgcggcaaccagatcggagccaagttctgggaagtgatcagcgatgaacatggaattgacccaaccggtacctaccacgg
ggacagcgacctgcagctggagagaataaacgtctattacaatgaggcaacgggtggcaagtacgtcccccgcgctgtgttggtcgatcttgaacccgga
accatggactccgtgcgctcagggccatttgggcagatctttaggcctgacaactttgtgtttggtcaaagcggtgctggaaacaactgggccaagggcc
actacacagagggagcagaactggtggactcagtgttggacgttgtgaggaaagaagcagaaagctgcgactgtctccagggcttccagctgacccactc
cctggggggcggcaccggctcgggcatgggcaccctgctcatcagcaaatcagggaagagtacccccgacagaatcatgaacactttcagcgtggtgccc
tcccccaaagtatctgacacagtagtagagccgtacaacgcccccctgtcggtgcatcagctggtaaaagaacccagaccgagacctactggtatagatt
aacgagggccctggtaagaaaatcctgctttcaaaaacccctcaaaactttaacaaccccccaccctaaggggggaaccttggaaaccacctttggggtc
ttcaggccccccccattgaagtgtggggggtaacacacaaccctggc
3' EST: CF783665 [+]tttttttttttttttatgaaaaatgcttttaatgaacggaacgttctgtacaactggtaacaatgaaaccggagagagaaggaaatgcaggaaacaaata
aaggaacgaacgtgagcagaaacagttgaataatttacagtagagatcagaagcttatgcattttcctcctcttccccttcctcaaactcgccctcctcc
tcggccgtggcatcctggtactgctggtactctgacaccaggtcgttcatgttgctctcggcctcggtgaactccatctcgtccatgccctcgcccgtgt
accagtgcaggaaggcctttcggcggaacttggcggtgaactgctcggagatgcgcttgaacagctcctggatggccgtgctgttgccgatgaaggtggc
ggacattttgaggcctcggggcgggatgtgtcgcagacggcggtcttcacgttgttggggatccattcgacgaagtagctgctgttcttgttctgcacgt
tcagcatctgctcatccacctccttcatggacatgcggcctcggaagacggcggccacggttcagtagcggccgtgacgggggtcgcaagcggccatcat
gttcttggcgtcgaacatctgctgggtgagcttctggaccggtaaaggcgcgggtactggctggctgccctctgctgggggaggggggggcaaacccggg
catgaaanaaatgccaaaccgggggaaaagggcccctggtttgaccggccaagttttgcgccggggcgggggtttcaactggccccaggggaaacccgca
ggggcggggtggggttaccccccccctctcgggggggggcttggaaaacaccaagaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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