XB-CLONE-4353353
Clone Name: IMAGE:7026134
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Gene:
atf4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783667 [+]
taattaatcccccccccccccgacgtcagtctgcagcttggaatgaaagagggaacgtagtttgctgtcgctgggtcttttttgttaactaaatactaag
gtttgaggacgcgacgcttctgctgtagcaggttccaacagtgatttcttcagtccggcgccgcaacaaccagcggacagccatggcgctatagcggcag
cacaggcctgcggctcccctctcccccggccatagcgaggcagcagctcccagcggcgcccgcagcaccaggcaacggcacagccatggcgcttctccag
gcatcccacagcagcattgctgtatacgaggaagaaagctcaaaattgtcatcaccagtcgaattcgtgatcctacagaaagatgagtttgttaagcgac
gagatgctgttgggggaccttctgtcccccttcaaccagtcgtttttggcggctgaaaagagtttgggtctcttggaggatgaatttgaggtgcccgggt
acctcctatcgcaagggttctccagcgacaaggctaaggatggcgtctctgacctgctgccttatgttgaatctttaggcagttttcatggggacgcctt
ctccggcatggattggatggtggaaaagatggatctgaaggaatttgatttagactccctgtttgggttggaagatctggaatccactatctcaccagat
gagctaacatccacgtttggaagactcatgtggatttccctanaagaaaccaccctcttgcagcaagtgatttgggcccactttggggccatcaccctga
aaccttgttctcctcttcccaaaaatca
3' EST: CF783666 [+]tttttttttttttttaactacaaaataatttattaagtagacattccacaacattcttaagtgacctatatacaacatacacacgctccagctttgttcc
ctattcaagaactttttgctcgtttgcttttggcctgtcggacttcagctatcagatctttcaaatactggatctcttttgttaacgaatctactttttc
tctaagggtatcattttttccctctagttctctgcactctccagatattaactcttgctctgctcttttcttctgtctgtagcgagtggctgctgttttg
ttctgctccattttctttttcttcttgtctactctgggctgccctccagctactttcaccttcgccatcacatctctatccttacagggaagatcataag
gtttgggtctctcagagactggggactctagggaggactgaggactactatagcattctacagttgatggactctgctgaggggattccaagtaggaggg
gctcatgctgattccactatcattatcagaggaatcttccttctcacatttaagtatctctagagtatatgttgcagaggactttttctcatcctctgca
acatctacctcactacctacatccaagatgaaggaatggtctgggctanaggagagagaggctgtcaaatctgggctaaatggggcaacctgatcagcat
ccagtggggactctggaaaagagacaggttcaggtgatggcacaaagtggccaatcactgctgcatgaggtgggtcctcttaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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